Sulfadiazine sodium
(Synonyms: 磺胺嘧啶钠) 目录号 : GC60347
A sulfonamide antibiotic
Cas No.:547-32-0
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Sulfadiazine is a sulfonamide antibiotic that inhibits the growth of Gram-positive and Gram-negative bacteria.1,2,3,4 It inhibits dihydropteroate synthase (DHPS), which converts a pteridine and 4-aminobenzoic acid to dihydropteroate, an intermediate in folate biosynthesis.1 Sulfadiazine inhibits recombinant P. carinii DHPS (IC50 = 0.19 μM). It is active against clinical isolates of M. tuberculosis (MIC90 = 10 mg/L) and of N. meningitidis (MICs = 5-2,000 mg/L).2,3 Sulfadiazine is also active against A. pleuropneumoniae, S. choleraesuis, S. typhimurium, P. multocida, S. equi, and S. suis (MIC90s = <0.5 mg/ml for all) when used in combination with trimethoprim .4 In vivo, sulfadiazine (40 mg/kg per day) increases survival in a mouse model of lethal T. gondii infection when administered in combination with pyrimethamine .5 Formulations containing sulfadiazine have been used in the treatment of rheumatic fever and various infections, and, in a dual treatment with pyrimethamine, to treat toxoplasmosis.
1.Hong, Y.-L., Hossler, P.A., Calhoun, D.H., et al.Inhibition of recombinant Pneumocystis carinii dihydropteroate synthetase by sulfa drugsAntimicrob. Agents Chemother.39(8)1756-1763(1995) 2.Ameen, S.M., and Drancourt, M.In Vitro Susceptibility of Mycobacterium tuberculosis to Trimethoprim and Sulfonamides in FranceAntimicrob. Agents Chemother.57(12)6370-6371(2013) 3.Wiggins, G.L., McLaughlin, J.V., Bickham, S.T., et al.Susceptibility of Neisseria meningitidis strains from the civilian population to sulfadiazine, penicillin, and rifampinAppl. Microbiol.20(6)893-898(1970) 4.Salmon, S.A., Watts, J.L., Case, C.A., et al.Comparison of MICs of ceftiofur and other antimicrobial agents against bacterial pathogens of swine from the United States, Canada, and DenmarkJ. Clin. Microbiol.33(9)2435-2444(1995) 5.Martins-Duarte, ?.S., de Souza, W., and Vommaro, R.C.Toxoplasma gondii: The effect of fluconazole combined with sulfadiazine and pyrimethamine against acute toxoplasmosis in murine modelExp. Parasitol.133(3)294-299(2013)
| Cas No. | 547-32-0 | SDF | |
| 别名 | 磺胺嘧啶钠 | ||
| Canonical SMILES | O=S(C1=CC=C(N)C=C1)(N([Na])C2=NC=CC=N2)=O | ||
| 分子式 | C10H9N4NaO2S | 分子量 | 272.26 |
| 溶解度 | DMSO: 125 mg/mL (459.12 mM) | 储存条件 | 4°C, protect from light, stored under nitrogen |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.673 mL | 18.3648 mL | 36.7296 mL |
| 5 mM | 0.7346 mL | 3.673 mL | 7.3459 mL |
| 10 mM | 0.3673 mL | 1.8365 mL | 3.673 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Sulfadiazine sodium Ameliorates the Metabolomic Perturbation in Mice Infected with Toxoplasma gondii
Antimicrob Agents Chemother 2019 Sep 23;63(10):e00312-19.PMID:31383652DOI:10.1128/AAC.00312-19.
In this study, we analyzed the global metabolomic changes associated with Toxoplasma gondii infection in mice in the presence or absence of Sulfadiazine sodium (SDZ) treatment. BALB/c mice were infected with T. gondii GT1 strain and treated orally with SDZ (250 μg/ml in water) for 12 consecutive days. Mice showed typical manifestations of illness at 20 days postinfection (dpi); by 30 dpi, 20% had survived and developed latent infection. We used ultraperformance liquid chromatography-mass spectrometry to profile the serum metabolomes in control (untreated and uninfected) mice, acutely infected mice, and SDZ-treated and infected mice. Infection induced significant perturbations in the metabolism of α-linolenic acid, purine, pyrimidine, arginine, tryptophan, valine, glycerophospholipids, and fatty acyls. However, treatment with SDZ seemed to alleviate the serum metabolic alterations caused by infection. The restoration of the serum metabolite levels in the treated mice was associated with better clinical outcomes. These data indicate that untargeted metabolomics can reveal biochemical pathways associated with restoration of the metabolic status of T. gondii-infected mice following SDZ treatment and could be used to monitor responses to SDZ treatment. This study provides a new systems approach to elucidate the metabolic and therapeutic effects of SDZ in the context of murine toxoplasmosis.
Exploration of synthesizing fluorescent silicon nanoparticles and label-free detection of Sulfadiazine sodium
Talanta 2020 Dec 1;220:121410.PMID:32928425DOI:10.1016/j.talanta.2020.121410.
Herein, silica nanoparticles (SiNPs) with blue-fluorescence have been originally synthesized through one facile hydrothermal way, and this kind of SiNPs were water-soluble with the relative quantum yield of around 6%. Meanwhile, N-(triethoxysilylpropyl) urea severed as the silica source, while potassium hydrogen phthalate as the doping reagent. Also, SiNPs exhibited the acceptable stability and excitation-dependent fluorescence property. Moreover, their surfaces of the obtained SiNPs were equipped with multiple functional groups including -Si-O-Si-, -Si-H, -COOH, -NH2 and -OH. Importantly, the fluorescence of SiNPs could be specifically quenched by Sulfadiazine sodium (SD-Na), thus achieving a label-free detection of SD-Na, which displayed a wide linear response in the range of 0.8 μM-800 μM with a detection limit of 1.02 μM. Additionally, we explored the mechanism of SiNPs sensing SD-Na on the basis of aggregation-induced quenching. To be specific, the particle size of SiNPs increased from 29.9 nm to 203.7 nm induced by the electrostatic interactions between SiNPs and SD-Na, which was further confirmed by high resolution transmission electron microscopy. Consequently, the proposed strategy here broadened the ways of assaying Sulfadiazine sodium.
Eco-toxic effects of Sulfadiazine sodium, sulfamonomethoxine sodium and enrofloxacin on wheat, Chinese cabbage and tomato
Ecotoxicology 2009 Oct;18(7):878-85.PMID:19554446DOI:10.1007/s10646-009-0349-7.
Investigation of the toxic effects of three veterinary drugs [Sulfadiazine sodium (SDS), sulfamonomethoxine sodium (SMMS), and enrofloxacin (EFLX)] on seed germination, root elongation and shoot elongation of wheat (Triticum aestivum L.), Chinese cabbage (Brassica campestris L.) and tomato (Cyphomandra betacea) was carried out. Significant linear relationships between the root and shoot elongation and the concentration of veterinary drugs addition were observed. The effects of the three veterinary drugs on seed germination of wheat, Chinese cabbages and tomato were not significant (P > 0.05), but on shoot and root elongation they were markedly significant (P < 0.05). The inhibitory rates of veterinary drugs on root and shoot elongation of crops were significantly stronger than that on seed germination. Based on IC(50) (drugs concentration when 50% plants show inhibition) of root elongation, wheat was the most sensitive plant to the toxicity of SDS with a IC(50) value as high as 28.1 mg/kg; Chinese cabbage was the most sensitive plant to the toxicity of SMMS with a IC(50) value as high as 27.1 mg/kg; tomato was the most sensitive plant to the toxicity of EFLX with a IC(50) value as high as 125.7 mg/kg. The toxic effects of Sulfadiazine sodium and sulfamonometh-oxine sodium on the three crops were much higher than that of enrofloxacin.
Efficient visible-light photocatalytic degradation of Sulfadiazine sodium with hierarchical Bi₇O₉I₃under solar irradiation
Water Sci Technol 2015;72(12):2122-31.PMID:26675999DOI:10.2166/wst.2015.433.
Bi₇O₉I₃, a kind of visible-light-responsive photocatalyst, with hierarchical micro/nano-architecture was successfully synthesized by oil-bath heating method, with ethylene glycol as solvent, and applied to degrade sulfonamide antibiotics. The as-prepared product was characterized by X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), UV-visible diffuse reflection spectra and scanning electron microscopy (SEM). XRD and XPS tests confirmed that the product was indeed Bi₇O₉I₃. The result of SEM observation shows that the as-synthesized Bi₇O₉I₃ consists of a large number of micro-sheets with parallel rectangle structure. The optical test exhibited strong photoabsorption in visible light irradiation, with 617 nm of absorption edges. Moreover, the difference in the photocatalytic efficiency of as-prepared Bi₇O₉I₃ at different seasons of a whole year was investigated in this study. The chemical oxygen demand removal efficiency and concentration of NO(3)(-) and SO(4)(2-) of solution after reaction were also researched to confirm whether degradation of the pollutant was complete; the results indicated a high mineralization capacity of Bi₇O₉I₃. The as-synthesized Bi₇O₉I₃exhibits an excellent oxidizing capacity of Sulfadiazine sodium and favorable stability during the photocatalytic reaction.
Molecular interaction of ctDNA and HSA with Sulfadiazine sodium by multispectroscopic methods and molecular modeling
Luminescence 2013 Sep-Oct;28(5):785-92.PMID:23322489DOI:10.1002/bio.2457.
Interactions of Sulfadiazine sodium (SD-Na) with calf thymus DNA (ctDNA) and human serum albumin (HSA) were studied using fluorescence spectroscopy, UV absorption spectroscopy and molecular modeling. The fluorescence experiments showed that the processes were static quenching. The results of UV spectra and molecular modeling of the interaction between SD-Na and ctDNA indicated that the binding mode might be groove binding. In addition, the interaction of SD-Na with HSA under simulative physiological conditions was also investigated. The binding constants (K) and the number of binding sites (n) at different temperatures (292, 302, 312 K) were 5.23 × 10(3) L/mol, 2.18; 4.50 × 10(3) L/mol, 2.35; and 4.08 × 10(3) L/mol, 2.47, respectively. Thermodynamic parameters including enthalpy change (ΔH) and entropy change (ΔS) were calculated, the results suggesting that hydrophobic force played a very important role in SD-Na binding to HSA, which was in good agreement with the molecular modeling study. Moreover, the effect of SD-Na on the conformation of HSA was analyzed using three-dimensional fluorescence spectra.