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Sulforhodamine B acid chloride Sale

(Synonyms: 丽丝胺碱性蕊香红B磺酰氯) 目录号 : GC30195

SulforhodamineBacidchloride是形成稳定共轭物的荧光蛋白标记物.

Sulforhodamine B acid chloride Chemical Structure

Cas No.:62796-29-6

规格 价格 库存 购买数量
100mg
¥1,071.00
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Sample solution is provided at 25 µL, 10mM.

Description

Sulforhodamine B acid chloride is a fluorescent protein label forming stable conjugates.

Sulforhodamine B (SRB) is often used as a membrane-impermeable polar tracer or used for cell density determination via determination of cellular proteins (cytotoxicity assay). The SRB assay has been used to inexpensively conduct various screening assays to investigate cytotoxicity in cell based studies. This method relies on the property of SRB, which binds stoichiometrically to proteins under mild acidic conditions and then can be extracted using basic conditions; thus, the amount of bound dye can be used as a proxy for cell mass, which can then be extrapolated to measure cell proliferation. The protocol can be divided into four main steps: preparation of treatment, incubation of cells with treatment of choice, cell fixation and SRB staining, and absorbance measurement. This assay is limited to manual or semiautomatic screening, and can be used in an efficient and sensitive manner to test chemotherapeutic drugs or small molecules in adherent cells. It also has applications in evaluating the effects of gene expression modulation (knockdown, gene expression upregulation), as well as to study the effects of miRNA replacement on cell proliferation[1].

[1]. Orellana EA, et al. Sulforhodamine B (SRB) Assay in Cell Culture to Investigate Cell Proliferation. Bio Protoc. 2016 Nov 5;6(21). pii: e1984.

化学性质

Cas No. 62796-29-6 SDF
别名 丽丝胺碱性蕊香红B磺酰氯
Canonical SMILES CCN(C1=CC2=[O+]C3=C(C=CC(N(CC)CC)=C3)C(C4=CC=C(S(=O)(Cl)=O)C=C4S(=O)([O-])=O)=C2C=C1)CC
分子式 C27H29ClN2O6S2 分子量 577.11
溶解度 DMSO : ≥ 100 mg/mL (173.28 mM);Water : 10 mg/mL (17.33 mM) 储存条件 Store at -20°C
General tips 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。
储备液的保存方式和期限:-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。
为了提高溶解度,请将管子加热至37℃,然后在超声波浴中震荡一段时间。
Shipping Condition 评估样品解决方案:配备蓝冰进行发货。所有其他可用尺寸:配备RT,或根据请求配备蓝冰。

溶解性数据

制备储备液
1 mg 5 mg 10 mg
1 mM 1.7328 mL 8.6639 mL 17.3277 mL
5 mM 0.3466 mL 1.7328 mL 3.4655 mL
10 mM 0.1733 mL 0.8664 mL 1.7328 mL
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计算

动物体内配方计算器 (澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系GLPBIO为您提供正确的澄清溶液配方)
% DMSO % % Tween 80 % saline
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