T-2 Tetraol
(Synonyms: T-2 四醇) 目录号 : GC44980
A metabolite of T-2 toxin
Cas No.:34114-99-3
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
T-2 Tetraol is a metabolite of T-2 toxin, and also a trichothecene mycotoxin, with less toxicity and is unable to induce apoptosis[1][2].
References:
[1]. Engler KH, et al. A colorimetric technique for detecting trichothecenes and assessing relative potencies. Appl Environ Microbiol. 1999 May;65(5):1854-7.
[2]. Islam Z, et al. Structure-function relationship of T-2 toxin and its metabolites in inducing thymic apoptosis in vivo in mice. Biosci Biotechnol Biochem. 1998 Aug;62(8):1492-7.
| Cas No. | 34114-99-3 | SDF | |
| 别名 | T-2 四醇 | ||
| Canonical SMILES | CC1=C[C@]2([H])[C@]([C@]([C@H](O)[C@H]3O)(C)[C@@]4(OC4)[C@]3([H])O2)(CO)C[C@@H]1O | ||
| 分子式 | C15H22O6 | 分子量 | 298.3 |
| 溶解度 | Dichloromethane: 10 mg/ml | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.3523 mL | 16.7616 mL | 33.5233 mL |
| 5 mM | 0.6705 mL | 3.3523 mL | 6.7047 mL |
| 10 mM | 0.3352 mL | 1.6762 mL | 3.3523 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Stability of T-2, HT-2, and T-2 Tetraol in biological fluids
J Anal Toxicol 1988 Jan-Feb;12(1):48-50.PMID:3352243DOI:10.1093/jat/12.1.48.
The stabilities of tritium-labeled T-2, HT-2, and T-2 Tetraol were studied in blood and urine at -70 degrees, 4 degrees, and 23 degrees C for 6 months in the presence of EDTA or NaF. Samples were counted with a radiochromatographic scanner and results indicated the stability of T-2 Tetraol greater than T-2 greater than HT-2. Toxins were most stable when stored at -70 degrees C, in the presence of NaF, and in urine (pH 6). They were less stable in saline (control, pH 7) and least stable in blood (pH 8). These results suggest that urine and T-2 Tetraol are the biological fluid and metabolite of choice for diagnostic purposes.
Pharmacokinetics of T-2 Tetraol, a urinary metabolite of the trichothecene mycotoxin, T-2 toxin, in dog
Xenobiotica 1987 Aug;17(8):941-50.PMID:3673109DOI:10.3109/00498258709044192.
1. The urinary metabolites of T-2 toxin were identified and analysed quantitatively after i.v. administration to dogs. 2. A new routine assay for T-2 Tetraol was developed and a pharmacokinetic study was carried out on this final hydrolytic metabolite of T-2 toxin. T-2 Tetraol was excreted in urine for 2-3 days. Its 'sigma minus' plot demonstrated a significantly longer apparent half-life than its precursors (T-2 toxin and HT-2 toxin). This fact was explained by extraplasma binding causing prolongation of the metabolism and excretion of T-2 toxin metabolites. 3. The urinary metabolites of T-2 toxin were: HT-2 toxin, T-2 triol and T-2 Tetraol. The metabolites were excreted in free and conjugated forms. In two dogs T-2 toxin was found in the urine in an amount which accounts for 3.2 and 16% of the administered dose respectively. The cumulative amount of the identified metabolites and toxins formed in the urine ranged from 9.7 to 17.3% in four dogs and 44.7% in one dog.
T-2 Tetraol is cytotoxic to a chicken macrophage cell line
Poult Sci 1997 Feb;76(2):311-3.PMID:9057211DOI:10.1093/ps/76.2.311.
Cytotoxic effects of T-2 Tetraol, a T-2 toxin derivative, on the MQ-NCSU chicken macrophage cell line were quantified by direct in vitro exposure. Macrophage cultures were exposed to 1, 10, 20, 40, 80, 160, and 320 micrograms/mL of T-2 Tetraol for 1 h. Macrophage viability after exposure to T-2 Tetraol. Macrophage viability was reduced by increasing concentrations of T-2 Tetraol (linear effect, P < or = 0.001; quadratic effect, P < or = 0.025). The ability of macrophages to adhere to glass surfaces was impaired by increasing concentrations of T-2 Tetraol (linear effect, P < or = 0.003). This experiment demonstrates that T-2 Tetraol is cytotoxic to chicken macrophages in vitro.
Differential association of T-2 and T-2 Tetraol with mammalian cells
J Pharmacol Exp Ther 1989 Sep;250(3):860-6.PMID:2778715doi
The interactions of T-2 and its metabolite T-2 Tetraol (hereafter tetraol) with CHO (Chinese hamster ovary cells) and CHO ribosomes were studied. T-2 was about 300-fold more potent at inhibiting protein synthesis in CHO than was tetraol. Association of T-2 with CHO was highly specific and achieved a maximum at a concentration producing complete inhibition of protein synthesis. Association of tetraol with CHO was of low specificity, but the specific fraction did correlate with the dose-response curve for protein synthesis inhibition. Binding of both T-2 and tetraol to isolated CHO ribosomes was quantitatively similar and highly specific. With isolated ribosomes, each toxin competed effectively for the binding of the other. Using intact cells, tetraol competed for T-2 cell association, but not the converse. The kinetics at physiological temperature for total and specific T-2 cell association were much more rapid than those for tetraol. Furthermore, the rate of tetraol-cell association was indistinguishable from the rate for cellular uptake of tritiated water. At 0 degrees C, there was a substantial association of T-2 with cells, whereas none was observed with tetraol. The kinetics of dissociation of both toxins from CHO were similar. We conclude that T-2 rapidly crosses the cell membrane of cells and binds to the intracellular target, the ribosomes. In contrast, tetraol is taken up by the cell much more slowly, and many more toxin molecules are found in the cell than there are ribosomes. It would appear that the main physical property of the toxins that brings about these results is the relative hydrophobicities of the molecules.(ABSTRACT TRUNCATED AT 250 WORDS)
Production and characterization of antibodies against HT-2 toxin and T-2 Tetraol tetraacetate
Appl Environ Microbiol 1987 Jan;53(1):17-21.PMID:3827243DOI:10.1128/aem.53.1.17-21.1987.
Three new immunogens which were prepared by conjugation of the carboxymethyl oxime (CMO) derivatives of HT-2 toxin, T-2 Tetraol (T-2 4ol), and T-2 Tetraol tetraacetate (T-2 4Ac) to bovine serum albumin (BSA) were tested for the production of antibodies against the major metabolites of T-2 toxin. Antibodies against HT-2 toxin and T-2 4Ac were obtained from rabbits 5 to 10 weeks after immunizing the animals with CMO-HT-2-BSA and CMO-T-2 4Ac-BSA conjugates. Immunization with CMO-T-2 4ol-BSA resulted in no antibody against T-2 4ol. The antibody produced against HT-2 toxin had great affinity for HT-2 toxin as well as good cross-reactivity with T-2 toxin. The relative cross-reactivities of anti-HT-2 toxin antibody with HT-2 toxin, T-2 toxin, iso-T-2 toxin, acetyl-T-2 toxin, 3'-OH HT-2, 3'-OH T-2, T-2 triol, and 3'-OH acetyl-T-2, were 100, 25, 10, 3.3, 0.25, 0.15, 0.12 and 0.08%, respectively. Antibody against CMO-T-2 4Ac was very specific for T-2 4Ac and had less than 0.1% cross-reactivity with T-2 toxin, HT-2 toxin, acetyl-T-2 toxin, diacetoxyscirpenol, deoxynivalenol, and deoxynivalenol triacetate as compared with T-2 4Ac. The detection limits for HT-2 toxin and T-2 4ol by radioimmunoassay were approximately 0.1 and 0.5 ng per assay, respectively.