T4 DNA
目录号 : GP22276T4 DNA Ligase Recombinant
Sample solution is provided at 25 µL, 10mM.
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| Purity | Source | Escherichia Colilambda lysogen NM 989. | |
| Phycical Appearance | Sterile filtered liquid formulation having a concentration of 167,000 U/ml. | Shipping Condition | Shipped with Ice Packs. |
| Synonyms | DNA ligase 4; EC 6.5.1.1; DNA ligase IV; Polydeoxyribonucleotide synthase [ATP] 4. | ||
| Biological Activity | One Weiss unit is equivalent to circa 67 cohesive-end ligation units.? T4 DNA Ligase is strongly inhibited by NaCl or KCl if the concentration is > 200mM.? Ligation of blunt-ended and single-base pair overhang fragments requires about 50 times as much enzyme to achieve the same extent of ligation as cohesive-end DNA fragments. Blunt-end ligation may be enhanced by addition of PEG 4000 (10% w/v final concentration) or hexamine chloride, or by reducing the ATP concentration to 50?M.? To dilute T4 DNA Ligase that will subsequently be stored at иC20°C , 50% glycerol storage buffer should be used; to dilute for immediate use, 1x T4 DNA Ligase reaction buffer can be used. | ||
| Formulation | 50mM Tris-HCl (pH 7.8 at 25°C), 10mM MgCl2, 10mM DTT, 1mM ATP, 25 µg/ml BSA and DNA (0.1 to 1 ?m in 5? termini). Optimal ligation occurs at 16C. | ||
One Weiss unit is equivalent to circa 67 cohesive-end ligation units.? T4 DNA Ligase is strongly inhibited by NaCl or KCl if the concentration is > 200mM.? Ligation of blunt-ended and single-base pair overhang fragments requires about 50 times as much enzyme to achieve the same extent of ligation as cohesive-end DNA fragments. Blunt-end ligation may be enhanced by addition of PEG 4000 (10% w/v final concentration) or hexamine chloride, or by reducing the ATP concentration to 50?M.? To dilute T4 DNA Ligase that will subsequently be stored at иC20°C , 50% glycerol storage buffer should be used; to dilute for immediate use, 1x T4 DNA Ligase reaction buffer can be used.
Cloning of restriction fragments.Joining linkers and adapters to blunt-ended DNA.