TAMRA phosphoramidite, 5-isomer
目录号 : GC59157
This phosphoramidite is used for synthesis of oligonucleotides 5'-labeled with TAMRA
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
This phosphoramidite is used for synthesis of oligonucleotides 5'-labeled with TAMRA.
TAMRA(carboxytetramethylrhodamine) is a xanthene dye from the rhodamine family with emission in the orangespectrum range (maximum at 563 nm). This fluorophore is traditionally used as a FRET-acceptor(and a quencher) in a pair with fluorescein (FAM) due to significant overlapping of theirspectra. Thus, this phosphoramidite is convenient for the synthesis of dual-labeled probes TaqMan, whichcontain 5'-terminal TAMRA and FAM in the middle of the sequence or at the 3'-end(using Fluorescein dT Phosphoramidite and FAMCPG, respectively).
TAMRA 5'-labeled oligonucleotides are commonly used for quantitative PCRand fragment analysis (for example, for microsatellite marker analysis) because the equipment available has a detectionchannel for TAMRA frequently.
The TAMRA dye is not stable in the presence of ammonium andsterically non-hindered primary amines, so it is strongly recommended to follow specifiedconditions for labeled oligonucleotide deprotection.
Usage
Coupling: 7.5 min.
Deprotection: tret-buthylamine : methanol : water 1 : 1 : 3 (v/v/v) («TAMRA cocktail») for 6 hours at 60 °C, then cool down to room temperature.
Due to complete and irreversible degradation of the TAMRA dye, do NOT use aqueous ammonium and AMA for deprotecting a modified oligonucleotide from the solid-phase support.
| Cas No. | SDF | ||
| 分子式 | C40H50N5O6P | 分子量 | 727.83 |
| 溶解度 | good in acetonitrile | 储存条件 | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.3739 mL | 6.8697 mL | 13.7395 mL |
| 5 mM | 0.2748 mL | 1.3739 mL | 2.7479 mL |
| 10 mM | 0.1374 mL | 0.687 mL | 1.3739 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。