Taurodeoxycholic Acid-d4 (sodium salt)
(Synonyms: Sodium Taurodeoxycholate-d4; TDCA-d4) 目录号 : GC19797
An internal standard for the quantification of taurodeoxycholic acid
Cas No.:2410279-82-0
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >99.00% deuterated forms (d1-d4)
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Taurodeoxycholic acid-d4 is intended for use as an internal standard for the quantification of taurodeoxycholic acid by GC- or LC-MS. Taurodeoxycholic acid is a taurine-conjugated form of the secondary bile acid deoxycholic acid [1] Taurodeoxycholic acid stimulates chloride ion secretion through calcium-activated chloride ion channels and cystic fibrosis transmembrane conductance regulator (CFTR) in Calu-3 airway epithelial cell monolayers when applied basolaterally.[2] Serum levels of taurodeoxycholic acid increase approximately 5-fold within two hours during an oral lipid tolerance test in humans.[1]
Reference:
[1]. Schmid, A., Neumann, H., Karrasch, T., et al. Bile acid metabolome after an oral lipid tolerance test by liquid chromatography-tandem mass spectrometry (LC-MS/MS). PLoS One 11(2), e0148869 (2016).
[2]. Hendrick, S.M., Mroz, M.S., Greene, C.M., et al. Bile acids stimulate chloride secretion through CFTR and calcium-activated Cl- channels in Calu-3 airway epithelial cells. Am. J. Physiol. Lung. Cell. Mol. Physiol. 307(5), L407-L418 (2014).
| Cas No. | 2410279-82-0 | SDF | |
| 别名 | Sodium Taurodeoxycholate-d4; TDCA-d4 | ||
| 化学名 | 2-[[(3α,5β,12α)-3,12-dihydroxy-24-oxocholan-24-yl-2,2,4,4-d4]amino]-ethanesulfonic acid, monosodium salt | ||
| Canonical SMILES | [H][C@]12[C@@]([C@@](CC[C@]3([H])[C@H](C)CCC(NCCS(=O)([O-])=O)=O)([H])[C@]3(C)[C@@H](O)C2)([H])CC[C@]4([H])C([2H])([2H])[C@H](O)C([2H])([2H])C[C@@]41C.[Na+] | ||
| 分子式 | C26H40D4NO6S • Na | 分子量 | 525.7 |
| 溶解度 | DMF: 25 mg/ml, DMSO: 20 mg/ml, Ethanol: 2 mg/ml | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.9022 mL | 9.5111 mL | 19.0223 mL |
| 5 mM | 0.3804 mL | 1.9022 mL | 3.8045 mL |
| 10 mM | 0.1902 mL | 0.9511 mL | 1.9022 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。