Taurolithocholic Acid-d4 MaxSpec® Standard
(Synonyms: Taurolithocholate-d4, TLCA-d4) 目录号 : GC48480
A quantitative analytical standard guaranteed to meet MaxSpec identity, purity, stability, and concentration specifications
Cas No.:2410279-96-6
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Taurolithocholic acid-d4 (TLCA-d4) is intended for use as an internal standard for the quantification of TLCA by GC- or LC-MS. TLCA is a taurine-conjugated form of the secondary bile acid lithocholic acid .1 TLCA (75 μM) increases caspase-3 and -7 activity in Hep3B cells transfected with sodium taurocholate cotransporting peptide (NTCP), but not nontransfected Hep3B cells.2 It has been used to induce cholestasis in ex vivo and in vivo animal models of hepatocellular cholestasis.3,4 Serum levels of TLCA increase approximately 5-fold within two hours during an oral lipid tolerance test in humans.1
TLCA-d4 MaxSpec® standard is a quantitative grade standard of TLCA-d4 that has been prepared specifically for mass spectrometry and related applications where quantitative reproducibility is required. The solution has been prepared gravimetrically and is supplied in a deactivated glass ampule sealed under argon. The concentration was verified by comparison to an independently prepared calibration standard. The verified concentration is provided on the certificate of analysis. This TLCA-d4 MaxSpec® standard is guaranteed to meet identity, purity, stability, and concentration specifications and is provided with a batch-specific certificate of analysis. Ongoing stability testing is performed to ensure the concentration remains accurate throughout the shelf life of the product. Note: The amount of solution added to the vial is in excess of the listed amount. Therefore, it is necessary to accurately measure volumes for preparation of calibration standards. Follow recommended storage and handling conditions to maintain product quality.
1.Schmid, A., Neumann, H., Karrasch, T., et al.Bile acid metabolome after an oral lipid tolerance test by liquid chromatography-tandem mass spectrometry (LC-MS/MS)PLoS One11(2)e0148869(2016) 2.Rust, C., Wild, N., Bernt, C., et al.Bile acid-induced apoptosis in hepatocytes is caspase-6-dependentJ. Biol. Chem.284(5)2908-2916(2009) 3.Denk, G.U., Maitz, S., Wimmer, R., et al.Conjugation is essential for the anticholestatic effect of NorUrsodeoxycholic acid in taurolithocholic acid-induced cholestasis in rat liverHepatology52(5)1758-1768(2010) 4.Javitt, N.B.Cholestasis in rats induced by taurolithocholateNature210(5042)1262-1263(1966)
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 2.0504 mL | 10.2522 mL | 20.5044 mL |
| 5 mM | 0.4101 mL | 2.0504 mL | 4.1009 mL |
| 10 mM | 0.205 mL | 1.0252 mL | 2.0504 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。