TBHQ (tert-Butylhydroquinone)
(Synonyms: 特丁基对苯二酚; tert-Butylhydroquinone) 目录号 : GC34057
TBHQ(tert-Butylhydroquinone)是一种强效的酚类抗氧化剂，能够减轻氧化应激和炎症反应。
Cas No.:1948-33-0
Sample solution is provided at 25 µL, 10mM.
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TBHQ(tert-Butylhydroquinone) is a powerful phenolic antioxidant capable of reducing oxidative stress and inflammatory reactions. It triggers the activation of Nrf2 and is recognized for its anti-inflammatory, anti-apoptotic, and neuroprotective properties[1-3].
TBHQ(tert-Butylhydroquinone) treatment(Pre-treating with 40μM) for 16 hours reduced paraquat (PQ)-induced apoptosis in cells [4]. TBHQ(tert-Butylhydroquinone) inhibits apoptosis and mitigates oxidative stress by activating the Nrf2/HO-1 pathway in HepG2 cells[5].
TBHQ(tert-Butylhydroquinone) (16.7mg/kg/d;daily for 5 days ;i.p) markedly ameliorated depression-like behavior induced by LPS and suppressed inflammatory responses along with microglial activation in mice [6]. TBHQ(tert-Butylhydroquinone) (50 mg/kg body weight in 1% DMSO; p.o; 14days) shields the testicular tissue and maintains steroidogenesis and spermatogenesis in rats treated with DOX by suppressing oxidative stress, inflammation, and apoptosis [7].
References:
[1]. Lu XY, Wang HD, et,al. Pretreatment with tert-butylhydroquinone attenuates cerebral oxidative stress in mice after traumatic brain injury. J Surg Res. 2014 May 1;188(1):206-12. doi: 10.1016/j.jss.2013.11.1106. Epub 2013 Dec 1. PMID: 24387843.
[2]. Bai J, Yu XJ, et,al. Tert-butylhydroquinone attenuates oxidative stress and inflammation in hypothalamic paraventricular nucleus in high salt-induced hypertension. Toxicol Lett. 2017 Nov 5;281:1-9. doi: 10.1016/j.toxlet.2017.08.018. Epub 2017 Aug 24. PMID: 28844481.
[3]. Sukumari-Ramesh S, Alleyne CH Jr. Post-Injury Administration of Tert-butylhydroquinone Attenuates Acute Neurological Injury After Intracerebral Hemorrhage in Mice. J Mol Neurosci. 2016 Apr;58(4):525-31. doi: 10.1007/s12031-016-0722-y. Epub 2016 Feb 11. PMID: 26867538.
[4]. Li H, Wu S, et,al. Neuroprotective effects of tert-butylhydroquinone on paraquat-induced dopaminergic cell degeneration in C57BL/6 mice and in PC12 cells. Arch Toxicol. 2012 Nov;86(11):1729-40. doi: 10.1007/s00204-012-0935-y. Epub 2012 Sep 15. PMID: 22983789.
[5]. Li R, Zhang P, et,al. Tert-butylhydroquinone mitigates Carbon Tetrachloride induced Hepatic Injury in mice. Int J Med Sci. 2020 Jul 29;17(14):2095-2103. doi: 10.7150/ijms.45842. PMID: 32922170; PMCID: PMC7484658.
[6]. Bian H, Yan F, et,al.Tert-butylhydroquinone prevents neuroinflammation and relieves depression via regulation of NLRP3 signaling in mice. Int Immunopharmacol. 2022 Jun;107:108723. doi: 10.1016/j.intimp.2022.108723. Epub 2022 Mar 23. PMID: 35338961.
[7]. Ujah GA, Nna VU, et,al. Tert-butylhydroquinone attenuates doxorubicin-induced dysregulation of testicular cytoprotective and steroidogenic genes, and improves spermatogenesis in rats. Sci Rep. 2021 Mar 9;11(1):5522. doi: 10.1038/s41598-021-85026-7. PMID: 33750916; PMCID: PMC7970903.
TBHQ(tert-Butylhydroquinone)是一种强效的酚类抗氧化剂，能够减轻氧化应激和炎症反应。它能激活Nrf2并有抗炎、抗凋亡和神经保护作用[1-3]。
40μM TBHQ(tert-Butylhydroquinone)预处理细胞16小时可减少百草枯(PQ)诱导的细胞凋亡[4]。TBHQ(tert-Butylhydroquinone)通过激活HepG2细胞Nrf2/HO-1通路抑制细胞凋亡，降低氧化应激[5]。
TBHQ(tert-Butylhydroquinone) (16.7mg/kg/d;daily for 5 days ;i.p)可显著改善LPS诱导的小鼠抑郁样行为，抑制炎症反应及小胶质细胞激活[6]。在DOX处理的大鼠中，TBHQ(tert-Butylhydroquinone) (50 mg/kg in 1% DMSO; p.o; 14days)通过抑制氧化应激、炎症和细胞凋亡来保护睾丸组织并维持类固醇生成和精子发生[7]。
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Cell experiment [1]: |
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Cell lines |
PC12 cells |
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Preparation method |
PC12 cells were incubated with or without 40 μM Tert-butylhydroquinone for 16 hours, followed by exposure to 100 / 300 μM paraquat (PQ) for 24 hours. |
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Reaction Conditions |
40 μM;16 h |
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Applications |
TBHQ(tert-Butylhydroquinone) pretreatment had protective effect on PQ-induced apoptosis. |
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Animal experiment [2]: |
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Animal models |
C57BL/6J male mice (weight 20-25g) |
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Preparation method |
LPS was administered intraperitoneally at a daily dose of 1 mg/kg for five consecutive days. TBHQ(tert-Butylhydroquinone), dissolved in dimethyl sulfoxide and then diluted with PBS, was injected intraperitoneally at a daily dose of 16.7 mg/kg for five days. |
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Dosage form |
16.7mg/kg/d;daily for 5 days ;i.p |
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Applications |
TBHQ(tert-Butylhydroquinone) ameliorated LPS-induced caspase-1 expression in mice. |
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References: [1]. Li H, Wu S, et,al. Neuroprotective effects of tert-butylhydroquinone on paraquat-induced dopaminergic cell degeneration in C57BL/6 mice and in PC12 cells. Arch Toxicol. 2012 Nov;86(11):1729-40. doi: 10.1007/s00204-012-0935-y. Epub 2012 Sep 15. PMID: 22983789. [2]. Bian H, Yan F, et,al. Tert-butylhydroquinone prevents neuroinflammation and relieves depression via regulation of NLRP3 signaling in mice. Int Immunopharmacol. 2022 Jun;107:108723. doi: 10.1016/j.intimp.2022.108723. Epub 2022 Mar 23. PMID: 35338961. |
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| Cas No. | 1948-33-0 | SDF | |
| 别名 | 特丁基对苯二酚; tert-Butylhydroquinone | ||
| Canonical SMILES | OC1=CC=C(O)C=C1C(C)(C)C | ||
| 分子式 | C10H14O2 | 分子量 | 166.22 |
| 溶解度 | DMSO : ≥ 56.66 mg/mL (340.87 mM) | 储存条件 | Store at RT |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 6.0161 mL | 30.0806 mL | 60.1612 mL |
| 5 mM | 1.2032 mL | 6.0161 mL | 12.0322 mL |
| 10 mM | 0.6016 mL | 3.0081 mL | 6.0161 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >99.50%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet