TGFA Human
目录号 : GP20942转化生长因子-α 人类重组体
Sample solution is provided at 25 µL, 10mM.
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| Purity | Greater than 95.0% as determined by SDS-PAGE. | Source | Escherichia Coli. |
| Phycical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. | Shipping Condition | Shipped at Room temp. |
| Synonyms | Transforming Growth Factor Alpha; Protransforming Growth Factor Alpha; TGF-Alpha; TGFA. | ||
| Amino Acid Sequence | VVSHFNDCPD SHTQFCFHGT CRFLVQEDKP ACVCHSGYVG ARCEHADLLA. | ||
| Solubility | It is recommended to reconstitute the lyophilized TGFA in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. | ||
| Stability | Lyophilized TGFA although stable at room temperature for 3 weeks, should be stored desiccated below -18°C . Upon reconstitution TGFA should be stored at 4°C between 2-7 days and for future use below -18°C .For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. | ||
| Biological Activity | The ED50, as measured in a proliferation assay using mouse BALB/c 3T3 cells, is 0.395ng/ml. | ||
| Formulation | Lyophilized from a 0.2µm filtered solution in 0.1% TFA. | ||
Transforming Growth Factor-Alpha (TGF-alpha) belongs to the EGF family of cytokines. TGFA soluble form is discharged from the membrane by proteolytic cleavage. Membrane-bound proTGF-alpha is biologically active and has a role in cell-cell adhesion or in the stimulation of adjacent cells. TGFA expression is common in transformed cells. Additionally, TGFA is expressed in normal tissues during embryogenesis and in adult cells/tissues, including the pituitary, keratinocytes, and macrophages.
The ED50, as measured in a proliferation assay using mouse BALB/c 3T3 cells, is 0.395ng/ml.
Lyophilized TGFA although stable at room temperature for 3 weeks, should be stored desiccated below -18°C . Upon reconstitution TGFA should be stored at 4°C between 2-7 days and for future use below -18°C .For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.