TGFB1 Human Recombinant
目录号 : GP20943转化生长因子-β 1 人类重组体
Sample solution is provided at 25 µL, 10mM.
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| Purity | Greater than 95.0% as determined by SDS-PAGE. | Source | CHO cells. |
| Phycical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. | Shipping Condition | Shipped at Room temp. |
| Synonyms | Transforming growth factor beta-1; TGF-beta-1; CED; DPD1; TGFB; TGF-b 1; LAP; TGFB1. | ||
| Amino Acid Sequence | ALDTNYCFSS TEKNCCVRQL YIDFRKDLGW KWIHEPKGYH ANFCLGPCPY IWSLDTQYSK VLALYNQHNP GASAAPCCVP QALEPLPIVY YVGRKPKVEQ LSNMIVRSCK CS. | ||
| Solubility | It is recommended to reconstitute the lyophilized TGFB1 in sterile 10mM HClat a concentration of 0.1 mg/ml, which can then be further diluted to other aqueous solutions. | ||
| Stability | Lyophilized TGFB1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C . Upon reconstitution TGFB1 Human should be stored at 4°C between 2-7 days and for future use below -18°C .For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. | ||
| Biological Activity | The ED50 as determined by the dose-dependent inhibition of IL-4-induced proliferation of HT-2 cells is 0.0149ng/ml, corresponding to a specific activity of 6.7x107units/mg. | ||
| Formulation | Lyophilized from a sterile filtered solution containing 0.1 % trifluoroacetic acid (TFA) And trehalose (1:20 protein to Trehalose ratio). | ||
Transforming growth factor betas (TGFBetas) mediate many cell-cell interactions that occur during embryonic development. Three TGFBetas have been identified in mammals. TGFBeta1, TGFBeta2 and TGFBeta3 are each synthesized as precursor proteins that are very similar in that each is cleaved to yield a 112 amino acid polypeptide that remains associated with the latent portion of the molecule.
The ED50 as determined by the dose-dependent inhibition of IL-4-induced proliferation of HT-2 cells is 0.0149ng/ml, corresponding to a specific activity of 6.7x107units/mg.
Lyophilized TGFB1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C . Upon reconstitution TGFB1 Human should be stored at 4°C between 2-7 days and for future use below -18°C .For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.