Theaflavin
(Synonyms: 茶黄素) 目录号 : GC32090
茶黄素(te黄素,TF)是一种首次从红茶中提取出来的独特的benzotropolones衍生物,具有抗氧化,自由基清除和金属螯合的功能。
Cas No.:4670-05-7
Sample solution is provided at 25 µL, 10mM.
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Theaflavin (TF) is a distinctive derivative of benzotropolones first extracted from black tea, and has antioxidant effects for its functions of radical scavenging and metal chelation [1,2]. Besides,theaflavin is a suitable natural inhibitor against influenza A (H1N1) neuraminidase[3].
Theaflavin(20 and 50 µg/ml;2 h) inhibited calcium oxalate monohydrate (COM)-induced renal HK-2 tubular epithelial cell oxidative stress injury[4]. Human umbilical vein endothelial cells (HUVECs) exposed to Theaflavin(0-200µM;24 h) can alleviate apoptosis and cell dysfunction induced by tert-butyl hydroperoxide (TBHP)[5]. Theaflavin(0-160µM;72 h) inhibited proliferation of CNE2 cells, promoted apoptosis, and up-regulated the expression of caspase 3, caspase 9, and Bax, and decreased the level of Bcl-2[6].
Theaflavin(20、50、100 mg/kg;14days;i. g.) alleviated CaOx nephrocalcinosis-induced oxidative stress injury and crystal deposition in mice[4]. In a renal ischemia/reperfusion(I/R) injury mouse model, theaflavin pretreatment(20/50/80 mg/kg;3days;i. g.) significantly inhibited I/R-induced kidney injury and cell apoptosis. It improved mitochondrial dysfunction by reducing mitochondrial damage and promoting mitochondrial membrane potential[7].
References:
[1]. Han X, Zhang J, et al. Theaflavin ameliorates ionizing radiation-induced hematopoietic injury via the NRF2 pathway. Free Radic Biol Med. 2017;113:59-70
[2]. San Cheang W, Yuen Ngai C, et al. Black tea protects against hypertension-associated endothelial dysfunction through alleviation of endoplasmic reticulum stress. Sci Rep. 2015;5:10340
[3]. Sahoo M, Jena L, et,al. Identification of Suitable Natural Inhibitor against Influenza A (H1N1) Neuraminidase Protein by Molecular Docking. Genomics Inform. 2016 Sep;14(3):96-103. doi: 10.5808/GI.2016.14.3.96. Epub 2016 Sep 30. PMID: 27729839; PMCID: PMC5056903.
[4]. Ye T, Yang X, et,al.Theaflavin protects against oxalate calcium-induced kidney oxidative stress injury via upregulation of SIRT1. Int J Biol Sci. 2021 Mar 2;17(4):1050-1060. doi: 10.7150/ijbs.57160. PMID: 33867828; PMCID: PMC8040307.
[5]. Chen D, Wu Z, et,al.Theaflavin Attenuates TBHP-Induced Endothelial Cells Oxidative Stress by Activating PI3K/AKT/Nrf2 and Accelerates Wound Healing in Rats. Front Bioeng Biotechnol. 2022 Mar 2;10:830574. doi: 10.3389/fbioe.2022.830574. PMID: 35309982; PMCID: PMC8924520.
[6]. Xu J, Wang SJ, et,al.Theaflavin promoted apoptosis in nasopharyngeal carcinoma unexpectedly via inducing autophagy in vitro. Iran J Basic Med Sci. 2022 Jan;25(1):68-74. doi: 10.22038/IJBMS.2021.59190.13143. PMID: 35656444; PMCID: PMC9118283.
[7]. Li Z, Zhu J, et,al. Theaflavin ameliorates renal ischemia/reperfusion injury by activating the Nrf2 signalling pathway in vivo and in vitro. Biomed Pharmacother. 2021 Feb;134:111097. doi: 10.1016/j.biopha.2020.111097. Epub 2020 Dec 16. PMID: 33341051.
茶黄素(te黄素,TF)是一种首次从红茶中提取出来的独特的benzotropolones衍生物,具有抗氧化,自由基清除和金属螯合的功能[1,2]。此外,茶黄素是一种合适的抗甲型H1N1流感神经氨酸酶的天然抑制剂[3]。
茶黄素(20和50 µg/ml;2 h)可抑制草酸钙(COM)诱导的肾HK-2小管上皮细胞氧化应激损伤[4]。人脐静脉内皮细胞(HUVECs)暴露于茶黄素(0-200µM;24 h)可减轻过氧化叔丁基(TBHP)诱导的细胞凋亡和功能障碍[5]。茶黄素(0 ~ 160µ m;72 h)抑制CNE2细胞增殖,促进凋亡,上调caspase 3、caspase 9、Bax表达,降低Bcl-2水平[6]。
茶黄素(20、50、100 mg/kg;14days;i. g.)减轻CaOx肾钙质沉着引起的小鼠氧化应激损伤和晶体沉积[4]。在肾缺血再灌注(I/R)损伤小鼠模型中,茶黄素预处理(20/50/80 mg/kg;3days;i. g.)显著抑制了肾损伤和细胞凋亡。它通过减少线粒体损伤和促进线粒体膜电位改善线粒体功能障碍[7]。
| Cell experiment [1]: | |
|
Cell lines |
HK-2 cell |
|
Preparation Method |
HK-2 cells were treated with or without Theaflavin (20 and 50 µg/ml) for 2 h followed by incubation with 100 mg/ml of calcium oxalate( CaOx ) monohydrate for 24 h. |
|
Reaction Conditions |
20 and 50 µg/ml Theaflavin;2 h |
|
Applications |
Theaflavin inhibited calcium oxalate monohydrate (COM)-induced renal HK-2 tubular epithelial cell oxidative stress injury. |
| Animal experiment [2]: | |
|
Animal models |
C57BL/6J mice(CaOx nephrocalcinosis model ) |
|
Preparation Method |
Mice were daily treated with TF by gavage at three different doses (20, 50, and 100 mg/kg) on days 1-14. |
|
Dosage form |
20、50、100 mg/kg Theaflavin;14days;i. g. |
|
Applications |
Theaflavin alleviated CaOx nephrocalcinosis-induced oxidative stress injury and crystal deposition in mice. |
|
References: [1].Ye T, Yang X, et,al.Theaflavin protects against oxalate calcium-induced kidney oxidative stress injury via upregulation of SIRT1. Int J Biol Sci. 2021 Mar 2;17(4):1050-1060. doi: 10.7150/ijbs.57160. PMID: 33867828; PMCID: PMC8040307. | |
| Cas No. | 4670-05-7 | SDF | |
| 别名 | 茶黄素 | ||
| Canonical SMILES | O=C1C(O)=CC([C@@H]2[C@H](O)CC3=C(O)C=C(O)C=C3O2)=CC4=C([C@@H]5[C@H](O)CC6=C(O)C=C(O)C=C6O5)C=C(O)C(O)=C41 | ||
| 分子式 | C29H24O12 | 分子量 | 564.49 |
| 溶解度 | Water : 2 mg/mL (3.54 mM);DMSO : 1.42 mg/mL (2.52 mM) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.7715 mL | 8.8576 mL | 17.7151 mL |
| 5 mM | 0.3543 mL | 1.7715 mL | 3.543 mL |
| 10 mM | 0.1772 mL | 0.8858 mL | 1.7715 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >99.50%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet