Thiamine nitrate (Vitamin B1 nitrate)
(Synonyms: 硝酸硫胺; Vitamin B1 nitrate) 目录号 : GC31482
A water-soluble vitamin
Cas No.:532-43-4
Sample solution is provided at 25 µL, 10mM.
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Thiamine is a water-soluble vitamin with antioxidant, neuroprotective, and anxiolytic properties.1,2,3,4 It inhibits lipid peroxidation in rat liver microsomes and free radical oxidation of oleic acid in vitro when used at concentrations ranging from 1 to 100 μM.1 In vivo, thiamine (100 mg/kg) reduces hepatic levels of aspartate aminotransferase (AST) and alanine aminotransferase (ALT), lipid peroxidation, and protein damage in a rat model of acute ethanol intoxication.2 It reverses predator stress-induced suppression of hippocampal neurogenesis and decreases the latency of step-down from a platform, indicating anxiolytic-like activity in mice.3 Thiamine (8.5 mg/100 g food) reduces neurodegeneration and increases survival in Slc19a3-/- mice, a model of thiamine metabolism dysfunction syndrome-2 (THMD-2).4
1.Lukienko, P.I., Mel'nichenko, N.G., Zverinskii, I.V., et al.Antioxidant properties of thiamineBull. Exp. Biol. Med.130(9)874-876(2000) 2.Portari, G.V., Ovidio, P.P., Deminice, R., et al.Protective effect of treatment with thiamine or benfotiamine on liver oxidative damage in rat model of acute ethanol intoxicationLife Sci.16221-24(2016) 3.Vignisse, J., Sambon, M., Gorlova, A., et al.Thiamine and benfotiamine prevent stress-induced suppression of hippocampal neurogenesis in mice exposed to predation without affecting brain thiamine diphosphate levelsMol. Cell. Neurosci.82126-136(2017) 4.Suzuki, K., Yamada, K., Fukuhara, Y., et al.High-dose thiamine prevents brain lesions and prolongs survival of Slc19a3-deficient micePloS One12(6)e0180279(2017)
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Animal experiment: |
Slc19a3 E314Q KI mice are maintained routinely with conventional diet, which has a Thiamine concentration (thiamine hydrochloride, MW=337.3) of 1.71 mg/100 g food. Two types of Thiamine-restrict food base on “purified diets for laboratory rodents” are prepared, in which Thiamine concentration is 0.60 mg/100 g food (35% Thiamine of conventional food) or 0.27 mg/100 g food (16% Thiamine of conventional food). A high-Thiamine-containing food is also prepared from AIN-93M, in which Thiamine concentration is five times that of CE-2 (thiamine: 8.50 mg/100 g food)[2]. |
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References: [1]. Kenneth Osiezagha, et al.Thiamine Deficiency and Delirium. Innov Clin Neurosci. 2013 Apr; 10(4): 26-32. |
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| Cas No. | 532-43-4 | SDF | |
| 别名 | 硝酸硫胺; Vitamin B1 nitrate | ||
| Canonical SMILES | CC1=C(CCO)SC=[N+]1CC2=CN=C(C)N=C2N.[O-][N+]([O-])=O | ||
| 分子式 | C12H17N5O4S | 分子量 | 327.36 |
| 溶解度 | DMSO : 15 mg/mL (45.82 mM) | 储存条件 | Store at 2-8°C, protect from light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.0547 mL | 15.2737 mL | 30.5474 mL |
| 5 mM | 0.6109 mL | 3.0547 mL | 6.1095 mL |
| 10 mM | 0.3055 mL | 1.5274 mL | 3.0547 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet