Thiazinamium chloride (Multergan chloride)
目录号 : GC31807
Thiazinamiumchloride化物具有有效的抗胆碱和抗过敏活性，并抑制TxB2合成其IC50值为0.2µM。
Cas No.:4320-13-2
Sample solution is provided at 25 µL, 10mM.
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Thiazinamium chloride possesses potent anticholinergic and antiallergic activity and inhibits TxB2 synthesis with IC50 value of 0.2 µM.
TxB2 synthesis by resting macrophages is inhibited by thiazinamium chloride and promethazine in a dose-dependent manner. Thiazinamium chloride inhibits TxBz synthesis but had no effect on the ingestion of zymosan particles. In contrast, chlorpromazine inhibits phagocytosis but not TxBz synthesis except at lo-3 M. Under the condition where indomethacin, a nown cyclooxygenase inhibitor, is inhibitory, promethazine but not thiazinamium chloride inhibits TxB2 synthesis from exogenous arachidonic acid. Treatment of macrophages with promethazine and chlorpromazine but not thiazinamium chloride results in a reduction in the oxidative burst during phagocytosis. Furthermore, the ability of thiazinamium chloride to selectively inhibit arachidonic acid metabolism may contribute to its bronchodilator/antiallergic activity[1].
[1]. Chang J, et al. Effects of thiazinamium chloride, promethazine and chlorpromazine on thromboxane B2 synthesis, phagocytosis and respiratory burst by rat alveolar macrophages. Biochem Pharmacol. 1983 Sep 15;32(18):2671-7.
Cell experiment: | Rats are anesthetized with an intraperitoneal injections of sodium pentobarbital (43mg/kg body wt) and exsanguinated by cardiac puncture; their lungs are isolated and lavaged with a total of 50mL of lavage solution. Lungs that exhibit infection or gross pathological changes are not used. The lavage fluid containing macrophages is centrifuged at 300g for 10 min, and the cell pellet is resuspended in serum free M199. Macrophage monolayers are established by incubating 1.5x106 cells in petri dishes (35x10mm) for 2 hr in an atmosphere of 95% room air and 5% carbon dioxide. After washing with I-IBSS to remove non-adherent cells, the cultures are incubated in serum-free Ml99 with or without zymosan (100μg/mL) in the absence or presence of drugs(including Thiazinamium chloride)[1]. |
References: [1]. Chang J, et al. Effects of thiazinamium chloride, promethazine and chlorpromazine on thromboxane B2 synthesis, phagocytosis and respiratory burst by rat alveolar macrophages. Biochem Pharmacol. 1983 Sep 15;32(18):2671-7. | |
| Cas No. | 4320-13-2 | SDF | |
| Canonical SMILES | C[N+](C)(C)C(C)CN1C2=C(C=CC=C2)SC3=CC=CC=C13.[Cl-] | ||
| 分子式 | C18H23ClN2S | 分子量 | 334.91 |
| 溶解度 | Soluble in DMSO | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.9859 mL | 14.9294 mL | 29.8588 mL |
| 5 mM | 0.5972 mL | 2.9859 mL | 5.9718 mL |
| 10 mM | 0.2986 mL | 1.4929 mL | 2.9859 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet