TMPD (hydrochloride)
(Synonyms: N,N,N′,N′-四甲基对苯二胺盐酸盐；四甲基对苯二胺二盐酸盐) 目录号 : GC45061
A reducing co-substrate for heme peroxidases
Cas No.:637-01-4
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
TMPD is an easily oxidizable compound that serves as a reducing co-substrate for heme peroxidases. TMPD undergoes one-electron oxidation by the heme peroxidase higher oxidation states (compounds I and II) producing a highly colored product that absorbs at 611 nm. Thus, the stoichiometry of oxidation is 2 moles of TMPD oxidized per mole of hydroperoxide reduced by the peroxidase. The extinction coefficient of the oxidized TMPD at 611 nm is 12,200. TMPD is also used for the detection of peroxidases on polyacrylamide gels.
| Cas No. | 637-01-4 | SDF | |
| 别名 | N,N,N′,N′-四甲基对苯二胺盐酸盐；四甲基对苯二胺二盐酸盐 | ||
| Canonical SMILES | CN(C)C1=CC=C(N(C)C)C=C1.Cl.Cl | ||
| 分子式 | C10H16N2•2HCl | 分子量 | 237.2 |
| 溶解度 | DMSO: 1.7 mg/ml,PBS (pH 7.2): 14.3 mg/ml | 储存条件 | Store at -20°C,protect from light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 4.2159 mL | 21.0793 mL | 42.1585 mL |
| 5 mM | 0.8432 mL | 4.2159 mL | 8.4317 mL |
| 10 mM | 0.4216 mL | 2.1079 mL | 4.2159 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Graphene oxide electrochemistry: the electrochemistry of graphene oxide modified electrodes reveals coverage dependent beneficial electrocatalysis
R Soc Open Sci 2017 Nov 15;4(11):171128.PMID:29291099DOI:10.1098/rsos.171128.
The modification of electrode surfaces is widely implemented in order to try and improve electron transfer kinetics and surface interactions, most recently using graphene related materials. Currently, the use of 'as is' graphene oxide (GO) has been largely overlooked, with the vast majority of researchers choosing to reduce GO to graphene or use it as part of a composite electrode. In this paper, 'as is' GO is explored and electrochemically characterized using a range of electrochemical redox probes, namely potassium ferrocyanide(II), N,N,N',N'-tetramethyl-p-phenylenediamine (TMPD), dopamine hydrochloride and epinephrine. Furthermore, the electroanalytical efficacy of GO is explored towards the sensing of dopamine hydrochloride and epinephrine via cyclic voltammetry. The electrochemical response of GO is benchmarked against pristine graphene and edge plane-/basal plane pyrolytic graphite (EPPG and BPPG respectively) alternatives, where the GO shows an enhanced electrochemical/electroanalytical response. When using GO as an electrode material, the electrochemical response of the analytes studied herein deviate from that expected and exhibit altered electrochemical responses. The oxygenated species encompassing GO strongly influence and dominate the observed voltammetry, which is crucially coverage dependent. GO electrocatalysis is observed, which is attributed to the presence of beneficial oxygenated species dictating the response in specific cases, demonstrating potential for advantageous electroanalysis to be realized. Note however, that crucial coverage based regions are observed at GO modified electrodes, owing to the synergy of edge plane sites and oxygenated species. We report the true beneficial electrochemistry of GO, which has enormous potential to be beneficially used in various electrochemical applications 'as is' rather than be simply used as a precursor to making graphene and is truly a fascinating member of the graphene family.
The fabrication, characterisation and electrochemical investigation of screen-printed graphene electrodes
Phys Chem Chem Phys 2014 Mar 14;16(10):4598-611.PMID:24458292DOI:10.1039/c3cp55435j.
We report the fabrication, characterisation (SEM, Raman spectroscopy, XPS and ATR) and electrochemical implementation of novel screen-printed graphene electrodes. Electrochemical characterisation of the fabricated graphene electrodes is undertaken using an array of electroactive redox probes and biologically relevant analytes, namely: potassium ferrocyanide(II), hexaammine-ruthenium(III) chloride, N,N,N',N'-tetramethyl-p-phenylenediamine (TMPD), β-nicotinamide adenine dinucleotide (NADH), L-ascorbic acid (AA), uric acid (UA) and dopamine hydrochloride (DA). The electroanalytical capabilities of the fabricated electrodes are also considered towards the sensing of AA and DA. The electrochemical and (electro)analytical performances of the fabricated screen-printed graphene electrodes are considered with respect to the relative surface morphologies and material compositions (elucidated via SEM, Raman, XPS and ATR spectroscopy), the density of electronic states (% global coverage of edge-plane like sites/defects) and the specific fabrication conditions utilised. Comparisons are made between two screen-printed graphene electrodes and alternative graphite based screen-printed electrodes. The graphene electrodes are fabricated utilising two different commercially prepared 'graphene' inks, which have long screen ink lifetimes (>3 hours), thus this is the first report of a true mass-reproducible screen-printable graphene ink. Through employment of appropriate controls/comparisons we are able to report a critical assessment of these screen-printed graphene electrodes. This work is of high importance and demonstrates a proof-of-concept approach to screen-printed graphene electrodes that are highly reproducible, paving the way for mass-producible graphene sensing platforms in the future.