TNF a Human, His
目录号 : GP20965Tumor Necrosis Factor-Alpha Human Recombinant, His Tag
Sample solution is provided at 25 µL, 10mM.
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| Purity | Greater than 97.0% as determined by SDS-PAGE. | Source | Escherichia Coli. |
| Phycical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. | Shipping Condition | Shipped at Room temp. |
| Synonyms | TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a; Cachectin; DIF; TNFA; TNFSF2. | ||
| Solubility | It is recommended to reconstitute the lyophilized TNF-ж┴ in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. | ||
| Stability | Lyophilized TNF-a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C . Upon reconstitution TNF-a should be stored at 4°C between 2-7 days and for future use below -18°C .For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. | ||
| Biological Activity | EC50 0.200-0.120ng/ml measured in a metabolic inhibitor actinomycin D assay using L-cells (ATCC CCL-1). | ||
| Formulation | TNF-a His is supplied in 1xPBS. | ||
Tumor necrosis factor is a cytokine involved in systemic inflammation and is a member of a group of cytokines that all stimulate the acute phase reaction. TNF is mainly secreted by macrophages. TNF causes apoptotic cell death, cellular proliferation, differentiation, inflammation, tumorigenesis and viral replication, TNF is also involved in lipid metabolism, and coagulation. TNF's primary role is in the regulation of immune cells.Dysregulation and, in particular, overproduction of TNF have been implicated in a variety of human diseases- autoimmune diseases, insulin resistance, and cancer.
EC50 0.200-0.120ng/ml measured in a metabolic inhibitor actinomycin D assay using L-cells (ATCC CCL-1).
Lyophilized TNF-a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C . Upon reconstitution TNF-a should be stored at 4°C between 2-7 days and for future use below -18°C .For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.