TNFSF12 Human
目录号 : GP21006TNF Ligand Superfamily Member 12 Human Recombinant
Sample solution is provided at 25 µL, 10mM.
Purity | Greater than 95% as determined by SDS-PAGE. | Source | Escherichia coli. |
Phycical Appearance | Filtered White lyophilized (freeze-dried) powder. | Shipping Condition | Shipped at Room temp. |
Synonyms | TWEAK; TNF-related weak inducer of apoptosis; TNFSF12; DR3LG; Apo3-Ligand; APO3L; TNFRSF12A; Tumor necrosis factor ligand superfamily member 12; MGC20669; MGC129581. | ||
Amino Acid Sequence | MHHHHHHRSA PKGRKTRARR AIAAHYEVHP RPGQDGAQAG VDGTVSGWEE ARINSSSPLR YNRQIGEFIV TRAGLYYLYC QVHFDEGKAV YLKLDLLVDG VLALRCLEEF SATAASSLGP QLRLCQVSGL LALRPGSSLR IRTLPWAHLK AAPFLTYFGL FQVH. | ||
Solubility | It is recommended to reconstitute the lyophilized TNFSF12 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. | ||
Stability | Lyophilized TNFSF12 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C . Upon reconstitution TWEAK should be stored at 4°C between 2-7 days and for future use below -18°C .For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. | ||
Biological Activity | The ED50, as measured in a proliferation assay using HUVECs, is less than 8ng/ml. | ||
Formulation | Lyophilized from a 0.2μm filtered solution in PBS. |
TNFSF12 is a cytokine that is part of the TNF ligand family. TNFSF12 is a ligand for the FN14/TWEAKR receptor. TNFSF12 has overlapping signaling functions with TNF, but displays a much wider tissue distribution. TNFSF12 induces apoptosis through multiple pathways of cell death in a cell type-specific manner. TNFSF12 promotes proliferation and migration of endothelial cells, and therefore acts as a regulator of angiogenesis. TNFSF12 is expressed in adult heart, pancreas, skeletal muscle, small intestine, spleen and peripheral blood lymphocytes. TWEAK h induces NFkB and chemokine secretion. TNFSF12 exerts an apoptotic activity in HT-29 human adenocarcinoma cells whilst cultured in the presence of IFN-?. TNFSF12 promotes proliferation and migration of endothelial cells.
The ED50, as measured in a proliferation assay using HUVECs, is less than 8ng/ml.
Lyophilized TNFSF12 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C . Upon reconstitution TWEAK should be stored at 4°C between 2-7 days and for future use below -18°C .For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.