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TNFSF12 Human

目录号 : GP21006

TNF Ligand Superfamily Member 12 Human Recombinant

TNFSF12 Human Chemical Structure

规格 价格 库存 购买数量
5μg
¥840.00
5-10工作日
25μg
¥2,030.00
5-10工作日
1mg
¥37,800.00
5-10工作日

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Sample solution is provided at 25 µL, 10mM.

Description

TNFSF12 Human Recombinant (94-249 a.a.) produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 156 amino acids and having a total molecular mass of 18kDa.The TNFSF12 is fused with an 8 amino acids his tag at N-terminal (M-HHHHHH-R, total 164 a.a.) and purified by proprietary chromatographic techniques.

Product Data

Purity Greater than 95% as determined by SDS-PAGE. Source Escherichia coli.
Phycical Appearance Filtered White lyophilized (freeze-dried) powder. Shipping Condition Shipped at Room temp.
Synonyms TWEAK; TNF-related weak inducer of apoptosis; TNFSF12; DR3LG; Apo3-Ligand; APO3L; TNFRSF12A; Tumor necrosis factor ligand superfamily member 12; MGC20669; MGC129581.
Amino Acid Sequence MHHHHHHRSA PKGRKTRARR AIAAHYEVHP RPGQDGAQAG VDGTVSGWEE ARINSSSPLR YNRQIGEFIV TRAGLYYLYC QVHFDEGKAV YLKLDLLVDG VLALRCLEEF SATAASSLGP QLRLCQVSGL LALRPGSSLR IRTLPWAHLK AAPFLTYFGL FQVH.
Solubility It is recommended to reconstitute the lyophilized TNFSF12 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability Lyophilized TNFSF12 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C . Upon reconstitution TWEAK should be stored at 4°C between 2-7 days and for future use below -18°C .For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Biological Activity The ED50, as measured in a proliferation assay using HUVECs, is less than 8ng/ml.
Formulation Lyophilized from a 0.2μm filtered solution in PBS.

Introduction

TNFSF12 is a cytokine that is part of the TNF ligand family. TNFSF12 is a ligand for the FN14/TWEAKR receptor. TNFSF12 has overlapping signaling functions with TNF, but displays a much wider tissue distribution. TNFSF12 induces apoptosis through multiple pathways of cell death in a cell type-specific manner. TNFSF12 promotes proliferation and migration of endothelial cells, and therefore acts as a regulator of angiogenesis. TNFSF12 is expressed in adult heart, pancreas, skeletal muscle, small intestine, spleen and peripheral blood lymphocytes. TWEAK h induces NFkB and chemokine secretion. TNFSF12 exerts an apoptotic activity in HT-29 human adenocarcinoma cells whilst cultured in the presence of IFN-?. TNFSF12 promotes proliferation and migration of endothelial cells.

Biological Activity

The ED50, as measured in a proliferation assay using HUVECs, is less than 8ng/ml.

Stability

Lyophilized TNFSF12 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C . Upon reconstitution TWEAK should be stored at 4°C between 2-7 days and for future use below -18°C .For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

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