TOP1 70kDa Human
目录号 : GP22309DNA Topoisomerase-I 70kDa Recombinant Human
Sample solution is provided at 25 µL, 10mM.
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| Purity | Greater than 95% as determined by SDS-PAGE. | Source | Sf9 insect cells. |
| Phycical Appearance | Shipping Condition | Shipped with Ice Packs. | |
| Synonyms | DNA topoisomerase 1; EC 5.99.1.2; DNA topoisomerase I; TOP1; Scl-70. | ||
| Stability | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.Avoid multiple freeze-thaw cycles. | ||
| Formulation | TOP1 70kDa is supplied in 20mM HEPES buffer pH-8.0, 500mM NaCl, and 20% glycerol. | ||
TOP1 is an important nuclear enzyme that interconverts supercoiled DNA to the necessary topological conformations for standard DNA replication and transcription. TOP1 is the target antigen for TOP1 autoantibodies. TOP1 antibodies are a specific marker in scleroderma patients (specificity 98-100%) and are related with the existence of diffuse skin involvement and pulmonary fibrosis. In human tissues top1 enzyme is primarily synthesized as a protein with a molecular weight of 100-kDa. Most of this precursor is then proteolytically processed to a 70-kDa size, from which the TOP1 antigen has derived its name.
Western blot with Scleroderma patient sera or monoclonal anti-hexa-His-tag antibody.
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.Avoid multiple freeze-thaw cycles.