trans-Zeatin
(Synonyms: 反-玉米素) 目录号 : GC30162
A cytokinin plant growth regulator
Cas No.:1637-39-4
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.50%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
trans-Zeatin is a cytokinin plant growth regulator with antioxidant and neuroprotective activities.1,2,3,4 It binds to the cytokinin receptor Arabidopsis histidine kinase 3 (AHK3) with a KD value of 1.3 nM.3 trans-Zeatin increases chlorophyll levels in etiolated Cucumus sativus cotyledons in a concentration-dependent manner.1 It increases callus growth and shoot formation in N. tabacum calluses when used at concentrations of 5 and 50 uM.2 trans-Zeatin (25-100 uM) reduces production of reactive oxygen species (ROS) induced by amyloid β (25-35) (Aβ25-35) in PC12 cells.4 It reduces scopolamine-induced spontaneous alternations in the Y-maze, indicating enhanced spatial memory, in mice when administered at doses of 1.5, 3, and 4.5 mg/kg per day.
1.Fletcher, R.A., and McCullagh, D.Cytokinin-induced chlorophyll formation in cucumber cotyledonsPlanta101(1)88-90(1971) 2.Yamada, Y., Sekiya, J., and Koshimizu, K.Cytokinin-induced shoot formationPhytochemistry11(3)1019-1021(1972) 3.Romanov, G.A., Lomin, S.N., and Schmülling, T.Biochemical characteristics and ligand-binding properties of Arabidopsis cytokinin receptor AHK3 compared to CRE1/AHK4 as revealed by a direct binding assayJ. Exp. Bot.57(15)4051-4058(2006) 4.Choi, S.J., Jeong, C.-H., Choi, S.-G., et al.Zeatin prevents amyloid β-induced neurotoxicity and scopolamine-induced cognitive deficitsJ. Med. Food12(2)271-277(2009)
| Cas No. | 1637-39-4 | SDF | |
| 别名 | 反-玉米素 | ||
| Canonical SMILES | OC/C(C)=C/CNC1=C2N=CNC2=NC=N1 | ||
| 分子式 | C10H13N5O | 分子量 | 219.24 |
| 溶解度 | DMSO : ≥ 60 mg/mL (273.67 mM) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 4.5612 mL | 22.8061 mL | 45.6121 mL |
| 5 mM | 0.9122 mL | 4.5612 mL | 9.1224 mL |
| 10 mM | 0.4561 mL | 2.2806 mL | 4.5612 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Photo- and Tetrazine-Responsive Modulation of trans-Zeatin
J Org Chem 2023 Mar 3;88(5):2921-2930.36763518 10.1021/acs.joc.2c02601
Photoirradiation and small organic molecule triggering of appropriately designed caged hormones enable the control and manipulation of the corresponding biological processes with high spatial and temporal resolution. Caged trans-Zeatin substituted with nitrobenzene carbonates as photoremovable protecting groups and trans-cyclooctene as the tetrazine-responsive motif have been synthesized. A smooth release of the trapped trans-Zeatin molecule has been achieved, permitting targeted perturbation of biological processes, including degradation, glucosylation, and recognition by appropriate enzymes.
trans-Zeatin induce regulation the biosynthesis of 2-acetyl-1-pyrroline in fragrant rice (Oryza sativa L.) seedlings
BMC Plant Biol 2023 Feb 11;23(1):88.36765297 PMC9921689
Background: In plants, cytokinin is activated into trans-Zeatin to fight abiotic stresses. However, the mechanism of the effect of trans-Zeatin on 2-acetyl-1-pyrroline (2-AP) biosynthesis in fragrant rice has yet to be studied. The present study was conducted to explore the effects of exogenous trans-Zeatin on enzymes activities, genes expression, and precursors involved in 2-AP biosynthesis and 2-AP contents as well as the seedling quality of a fragrant rice cultivar viz., Meixiangzhan2. Four concentrations of trans-Zeatin solutions at 20, 40, and 80 μmol L- 1 (ZT1, ZT2, and ZT3) were sprayed onto rice seedlings. Results: Compared to the control, trans-Zeatin treatments showed significantly higher 2-AP contents of fragrant rice seedlings. Increased plant height and stem width were observed due to trans-Zeatin treatments. The trans-Zeatin application increased 1-pyrroline, methylglyoxal, proline, and P5C contents, enhanced P5CS and OAT activities, and reduced glutamic acid contents. In addition, expressions of ProDH, P5CS2, and DAO4 were comparatively higher under trans-Zeatin treatments than CK in fragrant rice seedlings. Conclusions: Overall, up-regulation of P5C, 1-pyrroline, and proline and down-regulation of glutamic acid under appropriate trans-Zeatin concentrations (20 and 40 μmol L- 1) resulted in enhanced 2-AP biosynthesis in fragrant rice seedlings and 20-40 μmol L- 1 was considered as the suggested concentrations of trans-Zeatin application in fragrant rice seedling.
Aptamer-Based Colorimetric Probe for trans-Zeatin Detection Using Unmodified Gold Nanoparticle
Int J Anal Chem 2020 Oct 26;2020:8853451.33178280 PMC7609143
trans-Zeatin is the major active phytohormone in immature corn kernels. Herein, a highly sensitive, good selective and simple aptamer-based colorimetric method for the detection of trans-Zeatin was constructed. The selected aptamer sequence binds with trans-Zeatin and induces a duplex-to-aptamer structure switching. The gold nanoparticles (AuNPs) solution is stable with high-concentration salt, which is protected by red complementary DNA. In the absence of trans-Zeatin, the color of AuNPs changed from red to blue because aptamer DNA and complementary DNA form double-stranded DNA. Thus, the ratio of absorbance intensities (A522/A650) of AuNPs is changed with the concentration of trans-Zeatin. The color change could be observed by the naked eye. The linear range of this method covers a large variation of trans-Zeatin concentration from 0.05 to 0.75 μM. The detection limit is 0.037 μM. Moreover, this method was applied successfully to detect trans-Zeatin in real plant samples.
Endogenous trans-Zeatin content in plants with different metal-accumulating ability: a field survey
Environ Sci Pollut Res Int 2016 Dec;23(23):23422-23435.27613625 10.1007/s11356-016-7544-x
A field survey was conducted to evaluate soil metal pollution and endogenous trans-Zeatin content in the leaves of plants growing at six sites in a metal-polluted area located in Gejiu, Yunnan, China. Five plant species were collected, and the physicochemical properties and concentrations of five metals in the soil were analyzed. The trans-Zeatin content in plant leaves was measured by high-performance liquid chromatography. Based on the Nemerow pollution index, the six sites were classified into four levels of pollution (i.e., low, medium, high, and severely high). The degree of soil metal pollution was cadmium (Cd) > arsenic (As) > lead (Pb) > zinc (Zn) > copper (Cu). The leaf trans-Zeatin content in Pteris vittata (an arsenic hyperaccumulator) increased significantly by 98.6 % in soil with a severely high level of pollution compared with soil at a low level of pollution. However, in non-hyperaccumulators Bidens pilosa var. radiata and Ageratina adenophora, a significant decrease in leaf trans-Zeatin content of 35.6 and 87.6 %, respectively, was observed. The leaf trans-Zeatin content in Artemisia argyi also decreased significantly by 73.6 % in high metal-polluted soil compared with that in medium metal-polluted soil. Furthermore, significant correlations were observed between leaf trans-Zeatin content in Pteris vittata and As, Pb, and Cd concentrations in the soil; however, either no correlation or a negative one was observed in the other plant species. Therefore, a high content of trans-Zeatin in the leaves of Pteris vittata may play an important role in its normal growth and tolerance to metals.
A high trans-Zeatin nucleoside concentration in corms may promote the multileaf growth of Amorphophallus muelleri
Front Plant Sci 2022 Oct 6;13:964003.36275554 PMC9583388
Amorphophallus muelleri has a multileaf growth pattern different from that of other konjacs; however, the hormonal mechanism underlying this phenomenon is not clear. In this study, the levels of hormones closely related to the sprouting of the axillary bud, including five types of cytokinins, indole-3-acetic acid (IAA) and abscisic acid (ABA) were measured. In the second leaf sprouting stage, the content of trans-Zeatin riboside (tZR) in corms increased more than 5000-fold over that in the dormancy period. Surprisingly, although the expression of CYP735A1 and CYP735A2, which synthesize the precursors for tZR was elevated at the second leaf sprouting stage, the expression of IPTs, which have key roles in cytokinin biosynthesis, did not change significantly. In addition, most cytokinin contents in leaves during the same period were significantly lower than those in corms. We speculate that the high cytokinin contents in the corms may not biosynthesized de novo in corms. In addition, the IAA content in the corms also considerably increased during the second leaf sprouting stage. Indole-3-acetaldehyde oxidase (AO1) and auxin efflux carrier PIN1A, presented relatively high expression levels in the same period. In contrast, ABA content, and the expression of NCED1, a rate-limiting enzyme in ABA biosynthesis, were suppressed at the second leaf sprouting stage. It is worth mentioning that N6-(Δ2-isopentenyl) adenosine (iP)-type cytokinins have a high content in corms in the dormant period that significantly decreases after the first leaf sprouting stage, which is completely different from the trend of tZR. By treating dormant corms with iP, the percentage of multibud plants increased, and the growth performance in terms of bud and root length was significantly higher than those of the control. This implies that iP-type cytokinins tend to play a role in promoting first seedling sprouting. Furthermore, there was a remarkable increase of the IAA content in both corms and roots under iP treatment but an inhibitory effect in buds. We speculate that the increase in the IAA content induced by iP is tissue specific. These results will assist in the understanding of the role of hormones, especially cytokinins, in the multileaf growth type of konjac.