trans-Zeatin

trans-Zeatin is a cytokinin plant growth regulator with antioxidant and neuroprotective activities.^1,2,3,4 It binds to the cytokinin receptor Arabidopsis histidine kinase 3 (AHK3) with a K_D value of 1.3 nM.³ trans-Zeatin increases chlorophyll levels in etiolated Cucumus sativus cotyledons in a concentration-dependent manner.¹ It increases callus growth and shoot formation in N. tabacum calluses when used at concentrations of 5 and 50 uM.² trans-Zeatin (25-100 uM) reduces production of reactive oxygen species (ROS) induced by amyloid β (25-35) (Aβ25-35) in PC12 cells.⁴ It reduces scopolamine-induced spontaneous alternations in the Y-maze, indicating enhanced spatial memory, in mice when administered at doses of 1.5, 3, and 4.5 mg/kg per day.

1.Fletcher, R.A., and McCullagh, D.Cytokinin-induced chlorophyll formation in cucumber cotyledonsPlanta101(1)88-90(1971) 2.Yamada, Y., Sekiya, J., and Koshimizu, K.Cytokinin-induced shoot formationPhytochemistry11(3)1019-1021(1972) 3.Romanov, G.A., Lomin, S.N., and Schmülling, T.Biochemical characteristics and ligand-binding properties of Arabidopsis cytokinin receptor AHK3 compared to CRE1/AHK4 as revealed by a direct binding assayJ. Exp. Bot.57(15)4051-4058(2006) 4.Choi, S.J., Jeong, C.-H., Choi, S.-G., et al.Zeatin prevents amyloid β-induced neurotoxicity and scopolamine-induced cognitive deficitsJ. Med. Food12(2)271-277(2009)