trans-Zeatinriboside
(Synonyms: 反式玉米素核苷) 目录号 : GC30311A cytokinin
Cas No.:6025-53-2
Sample solution is provided at 25 µL, 10mM.
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trans-Zeatin riboside is a cytokinin that has been found in squash root xylem sap.1 It suppresses hypocotyl adventitious root formation in cucumber plants when used at concentrations ranging from 0.001 to 1 ?M. trans-Zeatin riboside (10 ?M) increases the activity of superoxide dismutase (SOD), ascorbate peroxidase (APX), catalase (CAT), and guaiacol peroxidase (POD) in the leaves, as well as improves turf quality and delays leaf wilting in a creeping bentgrass model of drought stress.2
1.Kuroha, T., Kato, H., Asami, T., et al.A trans-zeatin riboside in root xylem sap negatively regulates adventitious root formation on cucumber hypocotylsJ. Exp. Bot.53(378)2193-2200(2002) 2.Chang, Z., Liu, Y., Dong, H., et al.Effects of cytokinin and nitrogen on drought tolerance of creeping bentgrassPLos One11(4)e0154005(2016)
Cas No. | 6025-53-2 | SDF | |
别名 | 反式玉米素核苷 | ||
Canonical SMILES | C/C(CO)=C\CNC1=C(N=CN2[C@H]3[C@H](O)[C@H](O)[C@H](O3)CO)C2=NC=N1 | ||
分子式 | C15H21N5O5 | 分子量 | 351.36 |
溶解度 | DMSO : ≥ 100 mg/mL (284.61 mM) | 储存条件 | Store at -20°C |
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1 mg | 5 mg | 10 mg | |
1 mM | 2.8461 mL | 14.2304 mL | 28.4608 mL |
5 mM | 0.5692 mL | 2.8461 mL | 5.6922 mL |
10 mM | 0.2846 mL | 1.423 mL | 2.8461 mL |
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Inoculation of Azospirillum brasilense and exogenous application of trans-zeatin riboside alleviates arsenic induced physiological damages in wheat (Triticum aestivum)
Due to increased industrialization, arsenic (As) in the soil has become a serious issue for wheat production since past few decades. We investigated the role of Azospirillum brasilense and trans-zeatin riboside (tZR) in the mitigation of arsenic toxicity in wheat for 2 years (2018-2019 and 2019-2020) in pot experiments. Wheat plants grown in soil artificially spiked with arsenic (50, 70, and 100 μM) was left alone or amended with A. brasilense, tZR, or their combination as mitigation strategies. A treatment without arsenic or amendments was maintained as control. Arsenic-induced physiological damages were noticed in the wheat plants. Detrimental effects on the plant physiological functions, such as disruption of cell membrane stability, reduced water uptake, and stomatal functions, were noticed with increase in As toxicity. Application of biological amendments reversed the effects of As toxicity by increasing wheat plant growth rate, leaf area, and photosynthesis and also yield. Therefore, application of tZR and wheat seed inoculation with A. brasilense could be a sustainable and environmentally friendly strategy to mitigate arsenic-induced crop physiological damages.
Concentration Gradients of trans-Zeatin Riboside and trans-Zeatin in the Maize Stem: Measurement by a Specific Enzyme Immunoassay
A sensitive, specific enzyme immunoassay (EIA) for trans-zeatin riboside (ZR) and trans-zeatin (Z) in the 0.3 to 30 picomole range has been described. The reliability of the method for measuring ZR + Z in partially purified extracts of Zea mays L. tissues was verified by highperformance liquid chromatography. EIA measurements showed that there was a concentration gradient of ZR + Z along the length of the Zea stem. The topmost internodes, internodes 7 and 8 counting from the coleoptilar node, had the highest concentration ( approximately 130 picomoles per gram fresh weight). Moving basipetally, the concentration dropped approximately 10-fold to a minimum at internode 4, and then increased slightly in internodes 2 and 3. There were also gradients within each internode. The five lowest internodes contained the highest concentrations toward their apical end, the region which included the node; this asymmetry was less pronounced near the top of the plant.
Systemic transport of trans-zeatin and its precursor have differing roles in Arabidopsis shoots
Organ-to-organ signal transmission is essential for higher organisms to ensure coordinated biological reactions during metabolism and morphogenesis. Similar to organs in animals, plant organs communicate by various signalling molecules. Among them, cytokinins, a class of phytohormones, play a key role as root-to-shoot long-distance signals, regulating various growth and developmental processes in shoots1,2. Previous studies have proposed that trans-zeatin-riboside, a type of cytokinin precursor, is a major long-distance signalling form in xylem vessels and its action depends on metabolic conversion via the LONELY GUY enzyme in proximity to the site of action3-5. Here we report an additional long-distance signalling form of cytokinin: trans-zeatin, an active form. Grafting between various cytokinin biosynthetic and transportation mutants revealed that root-to-shoot translocation of trans-zeatin, a minor component of xylem cytokinin, controls leaf size but not meristem activity-related traits, whereas that of trans-zeatin riboside is sufficient for regulating both traits. Considering the ratio of trans-zeatin to trans-zeatin-riboside in xylem and their delivery rate change in response to environmental conditions, this dual long-distance cytokinin signalling system allows plants to fine-tune the manner of shoot growth to adapt to fluctuating environments.
A high trans-zeatin nucleoside concentration in corms may promote the multileaf growth of Amorphophallus muelleri
Amorphophallus muelleri has a multileaf growth pattern different from that of other konjacs; however, the hormonal mechanism underlying this phenomenon is not clear. In this study, the levels of hormones closely related to the sprouting of the axillary bud, including five types of cytokinins, indole-3-acetic acid (IAA) and abscisic acid (ABA) were measured. In the second leaf sprouting stage, the content of trans-zeatin riboside (tZR) in corms increased more than 5000-fold over that in the dormancy period. Surprisingly, although the expression of CYP735A1 and CYP735A2, which synthesize the precursors for tZR was elevated at the second leaf sprouting stage, the expression of IPTs, which have key roles in cytokinin biosynthesis, did not change significantly. In addition, most cytokinin contents in leaves during the same period were significantly lower than those in corms. We speculate that the high cytokinin contents in the corms may not biosynthesized de novo in corms. In addition, the IAA content in the corms also considerably increased during the second leaf sprouting stage. Indole-3-acetaldehyde oxidase (AO1) and auxin efflux carrier PIN1A, presented relatively high expression levels in the same period. In contrast, ABA content, and the expression of NCED1, a rate-limiting enzyme in ABA biosynthesis, were suppressed at the second leaf sprouting stage. It is worth mentioning that N6-(Δ2-isopentenyl) adenosine (iP)-type cytokinins have a high content in corms in the dormant period that significantly decreases after the first leaf sprouting stage, which is completely different from the trend of tZR. By treating dormant corms with iP, the percentage of multibud plants increased, and the growth performance in terms of bud and root length was significantly higher than those of the control. This implies that iP-type cytokinins tend to play a role in promoting first seedling sprouting. Furthermore, there was a remarkable increase of the IAA content in both corms and roots under iP treatment but an inhibitory effect in buds. We speculate that the increase in the IAA content induced by iP is tissue specific. These results will assist in the understanding of the role of hormones, especially cytokinins, in the multileaf growth type of konjac.
A trans-zeatin riboside in root xylem sap negatively regulates adventitious root formation on cucumber hypocotyls
Shoot cultures of cucumber were used to analyse the roles of root-derived substances in adventitious root formation on hypocotyl tissues. Xylem sap collected from the roots of squash had a strong inhibitory effect on the formation of hypocotyl adventitious roots. Double-solvent extraction followed by fractionation with both normal and reverse phase column chromatographies and analysis by liquid chromatography/tandem mass spectrometry identified trans-zeatin riboside (ZR) as the primary suppressor of adventitious root formation. ZR was the predominant cytokinin present in the xylem sap, occurring at a concentration of 2x10(-8 )M. Application of ZR at concentrations from 3.16x10(-9) M effected inhibition of adventitious root formation. These results suggest that ZR transported from roots via xylem sap may act as an endogenous suppressor of hypocotyl adventitious root formation in planta.