Trehalose 6-phosphate (potassium salt hydrate)
目录号 : GC48199An intermediate in the biosynthesis of trehalose
Sample solution is provided at 25 µL, 10mM.
Trehalose is a natural non-reducing disaccharide found in plants, invertebrates, fungi, and bacteria.1,2 In addition to roles as an energy source and stress protectant, trehalose can function as a signaling molecule.3,4 Trehalose 6-phosphate is an intermediate in the biosynthesis of trehalose. It is generated from UDP-glucose and glucose 6-phosphate by trehalose 6-phosphate synthase (TPS or otsA).5 In plants, changes in trehalose 6-phosphate levels positively correlate with diurnal changes in sucrose and may regulate diverse processes, including starch degradation and flowering.3,6
1.Walmagh, M., Zhao, R., and Desmet, T.Trehalose analogues: Latest insights in properties and biocatalytic productionInternational Journal of Molecular Sciences16(6)13729-13745(2015) 2.Goddijn, O.J., Verwoerd, T.C., Voogd, E., et al.Inhibition of trehalase activity enhances trehalose accumulation in transgenic plantsPlant Physiology113(1)181-190(1997) 3.Lunn, J.E., Delorge, I., Figueroa, C.M., et al.Trehalose metabolism in plantsPlant Journal79(4)544-567(2014) 4.Henry, C., Bledsoe, S.W., Siekman, A., et al.The trehalose pathway in maize: Conservation and gene regulation in response to the diurnal cycle and extended darknessJournal of Experimental Botany65(20)5959-5973(2014) 5.Padilla, L., KrÄmer, R., Stephanopoulos, G., et al.Overproduction of trehalose: Heterologous expression of Escherichia coli trehalose-6-phosphate synthase and trehalose-6-phosphate phosphatase in Corynebacterium glutamicumApplied and Environmental Microbiology70(1)370-376(2004) 6.Wahl, V., Ponnu, J., Schlereth, A., et al.Regulation of flowering by trehalose-6-phosphate signaling in Arabidopsis thalianaScience339(6120)704-707(2013)
| Cas No. | N/A | SDF | |
| Canonical SMILES | OC[C@@H](O1)[C@@H](O)[C@H](O)[C@@H](O)[C@@]1([H])O[C@@H]2[C@H](O)[C@@H](O)[C@H](O)[C@@H](COP([O-])([O-])=O)O2.[K+].[K+].O | ||
| 分子式 | C12H21O14P.2K [XH2O] | 分子量 | 498.5 |
| 溶解度 | Water: 50mg/mL | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.006 mL | 10.0301 mL | 20.0602 mL |
| 5 mM | 0.4012 mL | 2.006 mL | 4.012 mL |
| 10 mM | 0.2006 mL | 1.003 mL | 2.006 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet