Tributyrin
(Synonyms: 甘油三丁酸酯,Glyceryl tributyrate) 目录号 : GC61349
Glycerol tributyrate (Tributyrin) is a triglyceride that may inhibit cell growth and induce cell differentiation.
Cas No.:60-01-5
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Glycerol tributyrate (Tributyrin) is a triglyceride that may inhibit cell growth and induce cell differentiation.
| Cas No. | 60-01-5 | SDF | |
| 别名 | 甘油三丁酸酯,Glyceryl tributyrate | ||
| Canonical SMILES | CCCC(OCC(OC(CCC)=O)COC(CCC)=O)=O | ||
| 分子式 | C15H26O6 | 分子量 | 302.36 |
| 溶解度 | DMSO: 100 mg/mL (330.73 mM) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.3073 mL | 16.5366 mL | 33.0732 mL |
| 5 mM | 0.6615 mL | 3.3073 mL | 6.6146 mL |
| 10 mM | 0.3307 mL | 1.6537 mL | 3.3073 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Tributyrin administration improves intestinal development and health in pre-weaned dairy calves fed milk replacer
Anim Nutr 2022 Jun 21;10:399-411.PMID:35949196DOI:10.1016/j.aninu.2022.06.004.
Butyrate and its derivatives possess various nutritional and biological benefits for mammals, whereas its effects on dairy calves have not been well characterized. This study evaluated the effects of Tributyrin administration on blood immune, intestinal immune and barrier functions, and microbial composition of pre-weaned dairy calves. Twenty newborn Holstein bull calves were randomly assigned into a control group (no Tributyrin supplementation, CON; n = 10) or a treatment group (supplemented with Tributyrin at 2 g/L of milk, TRB; n = 10). The results showed that diarrhea frequency was decreased significantly by Tributyrin administration from d 29 to 56 (P < 0.001) and the whole period (P = 0.003, d 1 to 56) though no significant effects were observed on growth performance. For blood metabolites, Tributyrin administration significantly reduced the concentration of interleukin-1β (IL-1β) on d 28 (P = 0.001) and tended to reduce the concentration of serum amyloid A on d 56 (P = 0.079), whereas serum oxidative status parameters were not affected. For intestinal development, Tributyrin administration increased the villus height (P < 0.001) and the ratio of villus height to crypt depth (P = 0.046) in the jejunum, and the villus height in the ileum (P = 0.074). Furthermore, toll-like receptor 2 (TRL2, P = 0.045) and IL-1β (P = 0.088) gene expressions were downregulated, while claudin-4 (P = 0.022) gene expression was upregulated in the jejunum following Tributyrin administration. In the ileum, claudin-4 (P = 0.029) and G-protein coupled receptor 41 (P = 0.019) gene expressions were upregulated in the TRB group compared to CON. No significantly higher abundances of microbiota were found in the jejunum or ileum of calves in the CON group. In the TRB group, supplementing Tributyrin significantly increased the abundance of short-chain fatty acid (SCFA)-producing bacteria, including Ruminococcaceae, Lachnospiraceae, Prevotella and Rikenellaceae (LDA >3.5, P < 0.05), which was negatively associated with inflammatory gene expression (TLR2 and IL-1β) but positively associated with intestinal barrier genes (claudin-4) and morphological parameters (P < 0.05). In conclusion, supplementing Tributyrin in milk replacer could improve intestinal development and health of pre-weaned dairy calves by stimulating SCFA-producing bacteria colonization, enhancing intestinal barrier functions and suppressing inflammatory responses.
Tributyrin supplementation in pasteurized waste milk: Effects on growth performance, health, and blood parameters of dairy calves
J Dairy Sci 2021 Dec;104(12):12496-12507.PMID:34593232DOI:10.3168/jds.2021-20645.
This study evaluated the effects of incremental Tributyrin supplementation in pasteurized waste milk on growth performance, health, and blood metabolism of dairy calves before and after weaning. Forty-eight newborn female Holstein dairy calves (39.6 ± 2.75 kg; mean ± standard deviation) were blocked by age and randomly assigned to 3 treatments: pasteurized waste milk (1) without supplementation, (2) with 1 g/L of Tributyrin products (unprotected solid powder; containing 35% Tributyrin), or (3) with 2 g/L of Tributyrin products. The calves were weaned on d 56 and were raised until d 77. Data were analyzed for the preweaning, postweaning, and overall periods. The results showed that starter intake and hay intake were not different among treatments in any period of the trial, but the crude protein intake tended to increase linearly with Tributyrin supplementation during the overall period. Although Tributyrin supplementation had no effects on body weight during preweaning and overall periods, body weight increased linearly with Tributyrin supplementation postweaning. The average daily gain tended to increase linearly during postweaning and overall periods. No effects were observed on feed efficiency in any period. A positive linear relationship between body length and Tributyrin supplementation was observed during the postweaning period, but no differences were found for the other body structural measurements in any period. The results of diarrhea showed that Tributyrin concentration had a negative linear relationship with diarrhea frequency during preweaning and overall periods. The rectal temperature did not differ among treatments in any period, but a treatment × week effect for rectal body temperature was observed. For blood metabolism, Tributyrin supplementation had no effects on insulin, growth hormone, total protein, albumin, or globulin. No differences were found in serum amyloid A concentration in any of the periods, yet haptoglobin concentration decreased linearly with increasing Tributyrin concentration during postweaning and overall periods. Endothelin concentration showed a tendency to decrease linearly during preweaning and postweaning periods and decreased linearly with Tributyrin supplementation during the overall period. An increasing Tributyrin concentration was associated with a negative linear relationship with IL-1β concentration during the preweaning period, and no differences were found in the other periods. The concentration of IL-6 and tumor necrosis factor α were not different among treatments in any of the periods. These data suggest that increasing the concentration of Tributyrin in pasteurized waste milk could increase growth performance and health of dairy calves, and incremental Tributyrin supplementation could linearly reduce haptoglobin, endothelin, and IL-1β concentrations, indicating a positive effect of Tributyrin on alleviating oxidative stress and inflammatory status of dairy calves. Calves fed pasteurized waste milk supplemented with Tributyrin products (containing 35% Tributyrin) at 2 g/L compared with 1 g/L of milk had more improved growth and health.
Anticarcinogenic actions of Tributyrin, a butyric acid prodrug
Curr Drug Targets 2012 Dec;13(14):1720-9.PMID:23140283DOI:10.2174/138945012804545443.
Bioactive food compounds (BFCs) exhibit potential anticarcinogenic effects that deserve to be explored. Butyric acid (BA) is considered a promising BFC and has been used in clinical trials; however, its short half-life considerably restricts its therapeutic application. Tributyrin (TB), a BA prodrug present in milk fat and honey, has more favorable pharmacokinetic properties than BA, and its oral administration is also better tolerated. In vitro and in vivo studies have shown that TB acts on multiple anticancer cellular and molecular targets without affecting non-cancerous cells. Among the TB mechanisms of action, the induction of apoptosis and cell differentiation and the modulation of epigenetic mechanisms are notable. Due to its anticarcinogenic potential, strategies as lipid emulsions, nanoparticles, or structured lipids containing TB are currently being developed to improve its organoleptic characteristics and bioavailability. In addition, TB has minimal toxicity, making it an excellent candidate for combination therapy with other agents for the control of cancer. Despite the lack of data available in the literature, TB is a promising molecule for anticancer strategies. Therefore, additional preclinical and clinical studies should be performed using TB to elucidate its molecular targets and anticarcinogenic potential.
Dietary Tributyrin improves reproductive performance, antioxidant capacity, and ovary function of broiler breeders
Poult Sci 2021 Nov;100(11):101429.PMID:34555757DOI:10.1016/j.psj.2021.101429.
The objective of this experiment was to investigate the influence of dietary Tributyrin on reproduction performance and ovary function of broiler breeders with different egg laying rate. Two hundred fifty-six AA broiler breeders (48-wk-old) were allocated to 4 treatment in a 2 × 2 factorial arrangement with the main effects of Tributyrin supplementation (0 and 1,000 mg/kg Tributyrin [TRI]) and 2 egg laying rate levels (average [AR, 81.01 ± 0.79%] and low [LR, 70.98 ± 0.95%]). The results shown that the LR breeders presented higher egg weight, but lower egg laying rate, qualified egg rate and feed efficiency than the AR breeders (P(laying) < 0.05). Also, the superoxidase dismutase (SOD) activity in magnum was lower while malondialdehyde (MDA) was higher in ovary and magnum of LR breeders than that in the AR breeders (P(laying) < 0.05). Dietary supplementation with Tributyrin significantly enhanced egg weight (P(TRI) < 0.05), increased albumen height as well as Haugh unit (HU) in AR breeders (P(interaction) < 0.05), and also had higher total antioxidant capacity (T-AOC) and lower MDA in ovary (P(TRI) < 0.05). The cell apoptosis rate and proapoptosis related gene expression (caspase 8, 9 and Bax) in the ovary of LR breeders was higher, while anti-apoptosis related gene (Bcl-2) expression were lower in LR breeders when compared with the AR breeders (P(laying) < 0.05). Dietary supplementation with Tributyrin decreased the cell apoptosis rate and downregulated caspase 9 expression in LR breeders (P(Interaction) < 0.05), up-regulated the Bcl-2 expression in both 2 breeders (P(TRI) < 0.05). These findings suggest that the breeders with lower egg laying rate also characterized by deteriorate ovary function indicated by lower antioxidant capacity and higher cell apoptosis rate. Dietary supplementation with Tributyrin increased egg albumen quality, decreased ovarian proapoptosis related gene expression to improve reproductive tract function; and the positive effect on egg albumen quality is more pronounced in average reproductive breeders.
Oral gamma-cyclodextrin-encapsulated Tributyrin supplementation in young pigs with experimentally induced colitis
J Anim Sci 2022 Nov 1;100(11):skac314.PMID:PMC9671115DOI:10.1093/jas/skac314.
Disruption of intestinal integrity and barrier function due to tissue inflammation has negative implications on overall growth and well-being in young pigs. In this study, we investigated the effects of oral gamma-cyclodextrin-encapsulated Tributyrin (TBCD) in young pigs experiencing dextran sodium sulfate (DSS)-induced colitis. Pigs (n = 32 boars) were weaned from the sow at postnatal day (PND) 2, allotted to treatment based on the litter of origin and body weight (BW), and reared artificially over a 26-d feeding period. Treatment groups included: 1) nutritionally adequate (control) milk replacer, no DSS (Control n = 8), 2) control milk replacer plus oral DSS (DSS, n = 7), and 3) control diet supplemented with 8.3 g of TBCD per kg of reconstituted milk replacer plus oral DSS (TBCD + DSS, n = 8). Colitis was induced by administering DSS at 1.25 g of DSS/kg BW daily in a reconstituted milk replacer from PND 14-18. Milk replacer and water were provided ad libitum throughout the 26-d study. All the data were analyzed using a one-way ANOVA using the MIXED procedure of SAS. Control and DSS pigs had similar BW throughout the study, while TBCD + DSS pigs exhibited decreased (P < 0.05) BW starting at approximately PND 15. Additionally, average daily gain (ADG) before and after initiation of DSS dosing, along with over the total study duration, was decreased (P < 0.05) in pigs receiving TBCD + DSS compared with the Control. Milk disappearance was decreased (P < 0.05) in TBCD + DSS pigs when compared with Control and DSS groups. Both the concentration and molar ratio of cecal butyrate concentrations were increased (P < 0.05) in TBCD + DSS pigs compared with the Control group. The DSS and TBCD + DSS treatments also increased (P < 0.05) butyrate concentrations in the luminal contents with the proximal colon compared with Control. TBCD + DSS and DSS pigs had increased (P < 0.05) mucosal width in the distal colon compared with Control, thereby indicating heightened intestinal inflammation. Overall, oral supplementation of encapsulated Tributyrin increased the concentration of butyrate in the colon, but was unable to mitigate the negative effects of DSS-induced colitis.