Trolox
(Synonyms: 奎诺二甲基丙烯酸酯) 目录号 : GC19457
Vitamin E derivative with antioxidant properties
Cas No.:53188-07-1
Sample solution is provided at 25 µL, 10mM.
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Trolox is a vitamin E analogue and is a powerful antioxidant.
Trolox has shown to protect mammalian cells from oxidative damage. Trolox is effective in preventing myocyte necrosis in cell culture studies and in a canine model of two hours of left anterior descending coronary artery (LAD) occlusion followed by four hours of reperfusion[1]. Trolox could prevent oxidative stress-induced apoptosis in thymocytes. Pre- or posttreatment of cells with Trolox reduced H2O2-induced DNA fragmentation to control levels and below. [2]. Trolox is a hydrophilic analogue of alpha-tocopherol and reported to scavenge peroxyl radicals better than vitamin E in sodium dodecyl sulfate micelles and in liposomes. Trolox prolongs substantially the survival of human ventricular myocytes and hepatocyte against oxyradicals generated with xanthine oxidase plus hypoxanthine, and prevented lysis of red cells exposed to an azo-initiator[3].
Trolox是维生素E的类似物，是一种强效的抗氧化剂。
Trolox已经被证明可以保护哺乳动物细胞免受氧化损伤。在细胞培养研究和两个小时的左前降支冠状动脉（LAD）阻塞后四小时再灌注的犬模型中，Trolox能够有效预防肌细胞坏死[1]。Trolox可以预防氧化应激诱导的胸腺细胞凋亡。细胞的预处理或后处理使用Trolox将H2O2诱导的DNA断裂降至控制水平以下[2]。Trolox是α-生育酚的亲水性类似物，据报道在十二烷基硫酸钠胶束和脂质体中可以更好地清除过氧自由基。Trolox显著延长了人类室壁肌细胞和肝细胞在黄嘌呤氧化酶加上次黄嘌呤产生的氧自由基作用下的存活时间，并预防了暴露于偶氮引发剂下的红细胞的溶解[3]。
References:
[1]. Mickle DA, et al. Myocardial salvage with trolox and ascorbic acid for an acute evolving infarction. Ann Thorac Surg. 1989 Apr;47(4):553-7.
[2]. Forrest VJ, et al. Oxidative stress-induced apoptosis prevented by Trolox. Free Radic Biol Med. 1994 Jun;16(6):675-84.
[3]. Wu TW, et al. The cytoprotective effect of Trolox demonstrated with three types of human cells. Biochem Cell Biol. 1990 Oct;68(10):1189-94.
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Cell experiment: |
Thymocytes are treated with Trolox (10 mM) before, during, or after 10 min exposure to 10 μM H2O2. Trolox incubations that are conducted before or after H2O2 exposures are performed in TCM. Trolox treatments that are administered concurrently with H2O2 are performed in PBS with ferrous sulfate[1]. |
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References: [1]. Mickle DA, et al. Myocardial salvage with trolox and ascorbic acid for an acute evolving infarction. Ann Thorac Surg. 1989 Apr;47(4):553-7. |
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| Cas No. | 53188-07-1 | SDF | |
| 别名 | 奎诺二甲基丙烯酸酯 | ||
| Canonical SMILES | Cc1c2OC(C)(CCc2c(C)c(O)c1C)C(=O)O | ||
| 分子式 | C14H18O4 | 分子量 | 250.3 |
| 溶解度 | DMF: 30 mg/ml,DMSO: 20 mg/ml,Ethanol: 30 mg/ml,PBS (pH 7.2): 3 mg/ml | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.9952 mL | 19.976 mL | 39.9521 mL |
| 5 mM | 0.799 mL | 3.9952 mL | 7.9904 mL |
| 10 mM | 0.3995 mL | 1.9976 mL | 3.9952 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet