Trombodipine (PCA-4230)
(Synonyms: PCA-4230) 目录号 : GC32610
曲波地平 (PCA-4230) 是一种抗血栓剂。
Cas No.:113658-85-8
Sample solution is provided at 25 µL, 10mM.
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Trombodipine is an antithrombotic agent.
Trombodipine is an antithrombotic agent. Exposure to 50 μM Trombodipine reveals an important stasis of growth. The percentage of inhibition exerted by 1 μM Trombodipine on day 3, 5 and 7 of the growth curve is 22.0±4.5, 32.0±3.0 and 29.4±6.0%, respectively (P<0.05). The inhibitory effect of Trombodipine on cell proliferation is reversible and after removal of Trombodipine the proliferation of the cells is resumed[1]. Addition of Trombodipine reduces cell number in a dose-dependent manner over a period of 4 to 6 days in culture. When compare to control cultures at the latest time points investigated, 5 μM Trombodipine decreases the number of E19P cells and human VSMCs by 25 and 21%, respectively, whereas 50 μM Trombodipine reduces E19P and human VSMC number by 85 and 74%, respectively[2].
[1]. del Rio M, et al. Antiproliferative effects of PCA-4230, a new antithrombotic drug, in vascular smooth muscle cells. Br J Pharmacol. 1997 Apr;120(7):1360-6.
Cell experiment: | To estimate the proportions of cells in different phases of the cell cycle, cellular DNA contents are measured by flow cytometry. Cells are plated, allowed to attach overnight, and placed in serum-free medium for 48 h. Trombodipine (50 μM) is added at selected points during serum repletion. At the specified times after serum addition, the cells are harvested by trypsinization, washed with PBS, pelleted and resuspended in PBS containing 0.6% Nonidet P-40 and 100 μg/mL propidium iodide (PI). Flow cytometric analyses are done with a FACScan flow cytometer[1]. |
References: [1]. del Rio M, et al. Antiproliferative effects of PCA-4230, a new antithrombotic drug, in vascular smooth muscle cells. Br J Pharmacol. 1997 Apr;120(7):1360-6. | |
| Cas No. | 113658-85-8 | SDF | |
| 别名 | PCA-4230 | ||
| Canonical SMILES | O=C(C1=C(C)NC(C)=C(C(OCC)=O)C1C)OCCN(C(C2=C3C=CC=C2)=O)S3(=O)=O | ||
| 分子式 | C21H24N2O7S | 分子量 | 448.49 |
| 溶解度 | Soluble in DMSO | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.2297 mL | 11.1485 mL | 22.297 mL |
| 5 mM | 0.4459 mL | 2.2297 mL | 4.4594 mL |
| 10 mM | 0.223 mL | 1.1149 mL | 2.2297 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet