Trospium-d8 (chloride)
(Synonyms: 曲司氯铵 d8 (氯化物)) 目录号 : GC48209
A neuropeptide with diverse biological activities
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Trospium-d8 is intended for use as an internal standard for the quantification of trospium by GC- or LC-MS. Trospium is an antagonist of muscarinic (M) receptors (Kis = 0.50-2.3 nM for human M1-5 recombinant receptors, respectively).1 Formulations containing antimuscarinics, including trospium, are used to manage overactive bladder by relieving urgency, frequency, and incontinence.1,2,3
1.Hegde, S.S.Muscarinic receptors in the bladder: From basic research to therapeuticsBr. J. Pharmacol.147(2)S80-S87(2006) 2.Maggiore, U.L.R., Salvatore, S., Alessandri, F., et al.Pharmacokinetics and toxicity of antimuscarinic drugs for overactive bladder treatment in femalesExpert Opin.Drug Metab.Toxicol.8(11)1387-1408(2012) 3.Michel, M.C., and de la Rosette, J.J.M.C.H.Role of muscarinic receptor antagonists in urgency and nocturiaBJU Int.96(Suppl 1)37-42(2005)
| Cas No. | N/A | SDF | |
| 别名 | 曲司氯铵 d8 (氯化物) | ||
| Canonical SMILES | O=C(C(C1=CC=CC=C1)(O)C2=CC=CC=C2)OC3C[C@]([N+]45C([2H])([2H])C([2H])([2H])C([2H])([2H])C4([2H])[2H])([H])CC[C@@]5([H])C3.[Cl-] | ||
| 分子式 | C25H22D8NO3.Cl | 分子量 | 436 |
| 溶解度 | DMSO: soluble | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.2936 mL | 11.4679 mL | 22.9358 mL |
| 5 mM | 0.4587 mL | 2.2936 mL | 4.5872 mL |
| 10 mM | 0.2294 mL | 1.1468 mL | 2.2936 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Determination of the quaternary ammonium compound trospium in human plasma by LC-MS/MS: application to a pharmacokinetic study
J Chromatogr B Analyt Technol Biomed Life Sci 2010 Apr 15;878(13-14):981-6.PMID:20303326DOI:10.1016/j.jchromb.2010.02.028
A highly sensitive, specific and evaporation free SPE extraction, LC-MS/MS method has been developed for the estimation of trospium in human plasma using Trospium-d8 as an internal standard (IS). The analyte was separated using isocratic mobile phase on reverse phase column and analyzed by MS/MS in the multiple reaction monitoring mode using the respective [M(+)] cations, m/z 392-164 for trospium and m/z 400-172 for the IS. The total run time was 3.50 min and the elution of trospium and Trospium-d8 (IS) occurred at 2.8 min. The developed method was validated in human plasma with a lower limit of quantification of 0.05 ng/mL. A linear response function was established for the range of concentrations 0.05-10 ng/mL (r>0.998) for trospium in human plasma. The intra- and inter-day precision values for trospium met the acceptance as per FDA guidelines. Trospium was stable in the battery of stability studies viz., bench-top, auto-sampler, dry extracts and freeze/thaw cycles. The developed assay method was applied to an oral pharmacokinetic study in humans.