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Trypsin inhibitor(soybean) (≥4000 BAEE Units/mg) Sale

(Synonyms: 胰酶抑制剂) 目录号 : GC19839

Trypsin inhibitor(soybean)是一种胰蛋白酶(trypsin)抑制剂,IC50 为19 µM,也可以抑制胰凝乳蛋白酶,IC50 为14.3µM。

Trypsin inhibitor(soybean) (≥4000 BAEE Units/mg) Chemical Structure

Cas No.:9035-81-8

规格 价格 库存 购买数量
100mg
¥225.00
现货
500mg
¥480.00
现货
1g
¥900.00
现货
5g
¥3,570.00
现货

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Sample solution is provided at 25 µL, 10mM.

产品文档

Quality Control & SDS

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实验参考方法

Cell experiment [1]:

Cell lines

MCF-7、HepG2 cells

Preparation method

Different concentrations of the Trypsin inhibitor(soybean) (0-25µM) in 100µL complete RPMI medium was incubated with MCF-7 and HepG2 cells in a 96-well plate for 72 hours prior to incubation with MTT for 4 hours.

Reaction Conditions

0-25µM; 72 h

Applications

Trypsin inhibitor(soybean) inhibited proliferation of MCF-7 cells and HepG2 cells with IC50 values of 45.7µM and 23.7µM, respectively.

Animal experiment [2]:

Animal models

KM male mice

Preparation method

mice were divided into three groups randomly: the control group, Group Trypsin inhibitor (soybean) (control diet containing 2.0mg/g Trypsin inhibitor (soybean)), Group Trypsin inhibitor (soybean)+VC (Trypsin inhibitor (soybean) diet supplemented with 1500 mg/kg VC). A total of five intergroup parallels were set up for each group for the weekly sacrifice of animals. All the mice were sacrificed after a 5-week feeding. 

Dosage form

2.0mg/g; p.o.

Applications

Based on tissue section analysis, Trypsin inhibitor (soybean) causes irreversible damage to the pancreas. MDA in pancreatic mitochondria increased significantly in the experimental group and reached the maximum value (15.7 nmol/mg prot) in the third week.

References:

[1] Ye X, Ng T B. A trypsin-chymotrypsin inhibitor with antiproliferative activity from small glossy black soybeans[J]. Planta medica, 2009, 75(05): 550-556.

 

[2] Gu C, Yang Q, Li S, et al. Effects of Soybean Trypsin Inhibitor on Pancreatic Oxidative Damage of Mice at Different Growth Periods[J]. Foods, 2023, 12(8): 1691.

产品描述

Trypsin inhibitor(soybean) is a trypsin inhibitor with an IC50 of 19 µM. It can also inhibit chymotrypsin with an IC50 of 14.3µM[1]. Trypsin inhibitor (soybean) has strong binding force with trypsin and blocks its active site, Ki=1-10−5nM[2]. Trypsin inhibitor (soybean) can be added to cell culture medium to terminate trypsin digestion of cells [3]. Trypsin inhibitor (soybean) inhibits trypsin at a 1:1 molar ratio.

In vitro, Trypsin inhibitor (soybean) (0-25µM) treated MCF-7 and HepG2 cells for 72 hours, and both inhibited cell proliferation, with IC50 values of 45.7µM and 23.7µM respectively [1]. Trypsin inhibitor (soybean) (10μg/mL) treated HEK and A549 cells for 24 hours, which produced significant cytotoxic effects, was more toxic to A549 cells, and increased the activity of reactive oxygen species scavenging enzymes [4].

In vivo, Trypsin inhibitor (soybean) (2.0mg/g) was orally fed to KM mice for 5 weeks, which significantly increased pancreatic mitochondrial MDA content and reduced SOD and GSH-Px levels [5].

References:
[1] Ye X, Ng T B. A trypsin-chymotrypsin inhibitor with antiproliferative activity from small glossy black soybeans[J]. Planta medica, 2009, 75(05): 550-556.
[2] Blow D M, Janin J, Sweet R M. Mode of action of soybean trypsin inhibitor (Kunitz) as a model for specific protein–protein interactions[J]. Nature, 1974, 249(5452): 54-57.
[3] Stevenson N L, Bergen D J M, Lu Y, et al. Giantin is required for intracellular N-terminal processing of type I procollagen[J]. Journal of Cell Biology, 2021, 220(6): e202005166.
[4] Shamsi T N, Parveen R, Fatima S. Trypsin inhibitors demonstrate antioxidant activities, inhibit A549 cell proliferation, and increase activities of reactive oxygen species scavenging enzymes[J]. Indian Journal of Pharmacology, 2017, 49(2): 155-160.
[5] Gu C, Yang Q, Li S, et al. Effects of Soybean Trypsin Inhibitor on Pancreatic Oxidative Damage of Mice at Different Growth Periods[J]. Foods, 2023, 12(8): 1691.

Trypsin inhibitor(soybean)是一种胰蛋白酶(trypsin)抑制剂,IC50 为19 µM,也可以抑制胰凝乳蛋白酶,IC50 为14.3µM[1]。Trypsin inhibitor(soybean)与胰蛋白酶结合力很强,阻断其活性位点,Ki = 1-10−5nM[2]。Trypsin inhibitor(soybean)可用于添加到细胞培养基中终止胰蛋白酶消化细胞[3]。Trypsin inhibitor(soybean)以1:1的摩尔比抑制胰蛋白酶。

在体外,Trypsin inhibitor(soybean)(0-25µM)处理MCF-7和HepG2细胞72 h,均抑制了细胞的增殖,IC50值分别为45.7μM和23.7μM[1]。Trypsin inhibitor(soybean)(10μg/mL)处理HEK和A549细胞24h,产生了显著的细胞毒性作用,对A549细胞的毒性更强,并提高了活性氧清除酶的活性[4]

在体内,Trypsin inhibitor(soybean)(2.0mg/g)通过口服饲喂KM小鼠5周,显著升高了胰腺线粒体MDA含量,降低了SOD和GSH-Px水平[5]

Chemical Properties

Cas No. 9035-81-8 SDF
别名 胰酶抑制剂
分子式 分子量 ~22000
溶解度 H2O : 50 mg/mL (ultrasonic and warming and heat to 60°C) 储存条件 Store at 2-8°C,protect from light
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