Trypsin inhibitor(soybean) (≥4000 BAEE Units/mg)
(Synonyms: 胰酶抑制剂) 目录号 : GC19839
Trypsin inhibitor(soybean)是一种胰蛋白酶(trypsin)抑制剂，IC50 为19 µM，也可以抑制胰凝乳蛋白酶，IC50 为14.3µM。
Cas No.:9035-81-8
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
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Biological Activity: ≥4000 BAEE Units/mg
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Cell experiment [1]: | |
Cell lines | MCF-7、HepG2 cells |
Preparation method | Different concentrations of the Trypsin inhibitor(soybean) (0-25µM) in 100µL complete RPMI medium was incubated with MCF-7 and HepG2 cells in a 96-well plate for 72 hours prior to incubation with MTT for 4 hours. |
Reaction Conditions | 0-25µM; 72 h |
Applications | Trypsin inhibitor(soybean) inhibited proliferation of MCF-7 cells and HepG2 cells with IC50 values of 45.7µM and 23.7µM, respectively. |
Animal experiment [2]: | |
Animal models | KM male mice |
Preparation method | mice were divided into three groups randomly: the control group, Group Trypsin inhibitor (soybean) (control diet containing 2.0mg/g Trypsin inhibitor (soybean)), Group Trypsin inhibitor (soybean)+VC (Trypsin inhibitor (soybean) diet supplemented with 1500 mg/kg VC). A total of five intergroup parallels were set up for each group for the weekly sacrifice of animals. All the mice were sacrificed after a 5-week feeding. |
Dosage form | 2.0mg/g; p.o. |
Applications | Based on tissue section analysis, Trypsin inhibitor (soybean) causes irreversible damage to the pancreas. MDA in pancreatic mitochondria increased significantly in the experimental group and reached the maximum value (15.7 nmol/mg prot) in the third week. |
References: [1] Ye X, Ng T B. A trypsin-chymotrypsin inhibitor with antiproliferative activity from small glossy black soybeans[J]. Planta medica, 2009, 75(05): 550-556.
[2] Gu C, Yang Q, Li S, et al. Effects of Soybean Trypsin Inhibitor on Pancreatic Oxidative Damage of Mice at Different Growth Periods[J]. Foods, 2023, 12(8): 1691. | |
Trypsin inhibitor(soybean) is a trypsin inhibitor with an IC50 of 19 µM. It can also inhibit chymotrypsin with an IC50 of 14.3µM[1]. Trypsin inhibitor (soybean) has strong binding force with trypsin and blocks its active site, Ki=1-10−5nM[2]. Trypsin inhibitor (soybean) can be added to cell culture medium to terminate trypsin digestion of cells [3]. Trypsin inhibitor (soybean) inhibits trypsin at a 1:1 molar ratio.
In vitro, Trypsin inhibitor (soybean) (0-25µM) treated MCF-7 and HepG2 cells for 72 hours, and both inhibited cell proliferation, with IC50 values of 45.7µM and 23.7µM respectively [1]. Trypsin inhibitor (soybean) (10μg/mL) treated HEK and A549 cells for 24 hours, which produced significant cytotoxic effects, was more toxic to A549 cells, and increased the activity of reactive oxygen species scavenging enzymes [4].
In vivo, Trypsin inhibitor (soybean) (2.0mg/g) was orally fed to KM mice for 5 weeks, which significantly increased pancreatic mitochondrial MDA content and reduced SOD and GSH-Px levels [5].
References:
[1] Ye X, Ng T B. A trypsin-chymotrypsin inhibitor with antiproliferative activity from small glossy black soybeans[J]. Planta medica, 2009, 75(05): 550-556.
[2] Blow D M, Janin J, Sweet R M. Mode of action of soybean trypsin inhibitor (Kunitz) as a model for specific protein–protein interactions[J]. Nature, 1974, 249(5452): 54-57.
[3] Stevenson N L, Bergen D J M, Lu Y, et al. Giantin is required for intracellular N-terminal processing of type I procollagen[J]. Journal of Cell Biology, 2021, 220(6): e202005166.
[4] Shamsi T N, Parveen R, Fatima S. Trypsin inhibitors demonstrate antioxidant activities, inhibit A549 cell proliferation, and increase activities of reactive oxygen species scavenging enzymes[J]. Indian Journal of Pharmacology, 2017, 49(2): 155-160.
[5] Gu C, Yang Q, Li S, et al. Effects of Soybean Trypsin Inhibitor on Pancreatic Oxidative Damage of Mice at Different Growth Periods[J]. Foods, 2023, 12(8): 1691.
Trypsin inhibitor(soybean)是一种胰蛋白酶(trypsin)抑制剂，IC50 为19 µM，也可以抑制胰凝乳蛋白酶，IC50 为14.3µM[1]。Trypsin inhibitor(soybean)与胰蛋白酶结合力很强，阻断其活性位点，Ki = 1-10−5nM[2]。Trypsin inhibitor(soybean)可用于添加到细胞培养基中终止胰蛋白酶消化细胞[3]。Trypsin inhibitor(soybean)以1:1的摩尔比抑制胰蛋白酶。
在体外，Trypsin inhibitor(soybean)（0-25µM）处理MCF-7和HepG2细胞72 h，均抑制了细胞的增殖，IC50值分别为45.7μM和23.7μM[1]。Trypsin inhibitor(soybean)（10μg/mL）处理HEK和A549细胞24h，产生了显著的细胞毒性作用，对A549细胞的毒性更强，并提高了活性氧清除酶的活性[4]。
在体内，Trypsin inhibitor(soybean)（2.0mg/g）通过口服饲喂KM小鼠5周，显著升高了胰腺线粒体MDA含量，降低了SOD和GSH-Px水平[5]。
| Cas No. | 9035-81-8 | SDF | |
| 别名 | 胰酶抑制剂 | ||
| 分子式 | 分子量 | ~22000 | |
| 溶解度 | H2O : 50 mg/mL (ultrasonic and warming and heat to 60°C) | 储存条件 | Store at 2-8°C,protect from light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。