Tunicamycin Mixture
(Synonyms: 衣霉素) 目录号 : GC16738Tunicamycin Mixture是一种同源核苷抗生素的混合物, 可抑制N-糖基化并阻断GlcNAc 磷酸转移酶（GPT）。
Cas No.:11089-65-9
Sample solution is provided at 25 µL, 10mM.
Tunicamycin Mixture is a mixture of homologous nucleoside antibiotics that inhibits N-glycosylation and blocks GlcNAc phosphotransferase (GPT)[1]. Tunicamycin Mixture can induce cellular endoplasmic reticulum (ER) stress and lead to blockage of DNA synthesis and G1 phase arrest [2]. Tunicamycin Mixture inhibits Gram-positive bacteria, yeast, fungi and viruses and has anti-cancer activity [3].
In vitro, pretreatment of RAW264.7 cells with Tunicamycin Mixture (0.5μg/ml) for 2h significantly reduced LPS-induced nitrite production and weakened the expression of iNOS and COX-2 mRNA and protein[4]. Tunicamycin Mixture (1-20μg/ml) treated prostate cancer PC-3 cells for 24-96h, dose-dependently reduced cell viability, blocked the formation of N-glycosylation in proteins and induced endoplasmic reticulum stress [5] .
In vivo, Tunicamycin Mixture (1mg/kg) treated mice via intraperitoneal injection, significantly increased hepatic triglyceride accumulation, inhibited hepatic lipoprotein secretion, and reduced blood glucose levels and liver glycogen content [6]. The administration of a Tunicamycin mixture (2μg) via intratracheal delivery in a neutrophilic asthma model (OVALPS-OVA mice) increased the protein expression levels of endoplasmic reticulum stress markers and inflammatory cytokines, leading to a more severe asthma phenotype in the mice [7].
References:
[1] Hsu J L, Chiang P C, Guh J H. Tunicamycin induces resistance to camptothecin and etoposide in human hepatocellular carcinoma cells: role of cell-cycle arrest and GRP78[J]. Naunyn-Schmiedeberg's archives of pharmacology, 2009, 380: 373-382.
[2] Han C, Jin L, Mei Y, et al. Endoplasmic reticulum stress inhibits cell cycle progression via induction of p27 in melanoma cells[J]. Cellular signalling, 2013, 25(1): 144-149.
[3] Pałasz A, Cież D. In search of uracil derivatives as bioactive agents. Uracils and fused uracils: Synthesis, biological activity and applications[J]. European journal of medicinal chemistry, 2015, 97: 582-611.
[4] Kim S Y, Hwang J S, Han I O. Tunicamycin inhibits Toll-like receptor-activated inflammation in RAW264. 7 cells by suppression of NF-κB and c-Jun activity via a mechanism that is independent of ER-stress and N-glycosylation[J]. European journal of pharmacology, 2013, 721(1-3): 294-300.
[5] Guha P, Kaptan E, Gade P, et al. Tunicamycin induced endoplasmic reticulum stress promotes apoptosis of prostate cancer cells by activating mTORC1[J]. Oncotarget, 2017, 8(40): 68191.
[6] Feng B, Huang X, Jiang D, et al. Endoplasmic reticulum stress inducer tunicamycin alters hepatic energy homeostasis in mice[J]. International journal of molecular sciences, 2017, 18(8): 1710.
[7]Guo Q, Li H, Liu J, et al. Tunicamycin aggravates endoplasmic reticulum stress and airway inflammation via PERK-ATF4-CHOP signaling in a murine model of neutrophilic asthma[J]. Journal of Asthma, 2017, 54(2): 125-133.
Cell experiment [1]: | |
Cell lines | RAW264.7 cells |
Preparation method | RAW264.7 cells were pre-treated with 0.5μg/ml Tunicamycin Mixture for 2h and then incubated with LPS for 18h. |
Reaction Conditions | 0.5μg/ml; 2 h |
Applications | Tunicamycin Mixture significantly reduced LPS-induced nitrite production/release and attenuated iNOS and COX-2 mRNA and protein expression. |
Animal experiment [2]: | |
Animal models | C57BL/6 male mice |
Preparation method | The mice were randomly divided into 2 groups according to similar average body weight and blood glucose level. One group were injected intraperitoneally with 1mg/kg Tunicamycin Mixture or vehicle. The body weight and blood glucose level at fed state were measured 24 h after injection. Later, mice were euthanized using carbon dioxide, followed by cervical dislocation. |
Dosage form | 1mg/kg；i.p. |
Applications | Tunicamycin Mixture treatment for 24 hours significantly increased liver triglyceride accumulation, inhibited liver lipoprotein secretion, and reduced blood sugar levels and liver glycogen content. |
References: [1] Kim S Y, Hwang J S, Han I O. Tunicamycin inhibits Toll-like receptor-activated inflammation in RAW264. 7 cells by suppression of NF-κB and c-Jun activity via a mechanism that is independent of ER-stress and N-glycosylation[J]. European journal of pharmacology, 2013, 721(1-3): 294-300. [2] Feng B, Huang X, Jiang D, et al. Endoplasmic reticulum stress inducer tunicamycin alters hepatic energy homeostasis in mice[J]. International journal of molecular sciences, 2017, 18(8): 1710. | |
| Cas No. | 11089-65-9 | SDF | |
| 别名 | 衣霉素 | ||
| Canonical SMILES | O=C1C=CN([C@@H]2O[C@H]([C@H](O)C[C@H]3O[C@@H](O[C@@H]4[C@H](NC(C)=O)[C@@H](O)[C@H](O)[C@@H](CO)O4)[C@H](NC(/C=C/[(CH2)n]C(C)C)=O)[C@@H](O)[C@H]3O)[C@@H](O)[C@H]2O)C(N1)=O | ||
| 分子式 | C39H64N4O16 (for Tunicamycin VII) | 分子量 | 845.0 |
| 溶解度 | 20mg/mL in DMSO or DMF | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.1834 mL | 5.9172 mL | 11.8343 mL |
| 5 mM | 0.2367 mL | 1.1834 mL | 2.3669 mL |
| 10 mM | 0.1183 mL | 0.5917 mL | 1.1834 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
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