type I hair keratin fragment [Homo sapiens]/[Ovis aries]/[Rattus norvegicus]
(Synonyms: H2N-Leu-Asn-Asp-Arg-Leu-Ala-Ser-Tyr-Leu-OH ) 目录号 : GP10050
type I hair keratin
![type I hair keratin fragment [Homo sapiens]/[Ovis aries]/[Rattus norvegicus] Chemical Structure](/media/struct/GP1/GP10050.png "type I hair keratin fragment [Homo sapiens]/[Ovis aries]/[Rattus norvegicus] Chemical Structure")
Sample solution is provided at 25 µL, 10mM.
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The human type I hair keratin subfamily comprises nine individual members, which can be subdivided into three groups. Group A (hHa1, hHa3-I, hHa3-II, hHa4) and B (hHa7, hHa8) each contains structurally related hair keratins, whereas group C members hHa2, hHa5, and hHa6 represent structurally rather unrelated hair keratins1.
Keratins are divided into type I (acidic) and type II (basic to neutral) members, which form the 10-nm intermediate filament cytoskeletal network of epithelial cells through obligatory association of equimolar amounts of type I and type II keratins2,3,4.
The various type I hair keratin antibodies proved to be highly specific in that they recognized only single protein bands when analyzed in Western blots of one dimensionally resolved human hair keratins. One exception concerned the hHa2 antibody, which besides the prominent hHa2 protein band also reacted with a slightly larger minor band1. . A LEF-1 consensus sequence is invariably located at a highly conserved position in the proximal promoter region of the members of the human type I hair keratin family but it is specifically lacking in the entire promoter of the hHa7 gene5.
References:
1. L. Langbein, M. A. Rogers et al. The Catalog of Human Hair Keratins. The journal of biological chemistry. 274: 19874-19884, 1999
2.Steinert, P. M., and Roop, D. R. (1988) Annu. Rev. Biochem. 57, 593-625.
3.Fuchs, E., and Weber, K. (1994) Annu. Rev. Biochem. 63, 345-382.
4.Mischke, D. (1998) in Intermediate Filaments: Subcellular Biochemistry (Herrmann, H., and Harris, J. R., eds) Vol. 31, pp. 71-95, Plenum Press, New York.
5.Rogers, M. A., Winter, H., Wolf, C., Jacobs, M., and Schweizer, J. (1998)J. Biol. Chem. 273, 26683-26691.
| Cas No. | SDF | ||
| 别名 | H2N-Leu-Asn-Asp-Arg-Leu-Ala-Ser-Tyr-Leu-OH | ||
| Canonical SMILES | NC(CC(C)C)C(NC(CC(N)=O)C(NC(CC(O)=O)C(NC(CCCNC(N)=N)C(NC(CC(C)C)C(NC(C)C(NC(CO)C(NC(CC1=CC=C(O)C=C1)C(NC(CC(C)C)C(O)=O)=O)=O)=O)=O)=O)=O)=O)=O | ||
| 分子式 | C47H77N13O15 | 分子量 | 1064.19 |
| 溶解度 | ≥ 106.4mg/mL in DMSO | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 0.9397 mL | 4.6984 mL | 9.3968 mL |
| 5 mM | 0.1879 mL | 0.9397 mL | 1.8794 mL |
| 10 mM | 0.094 mL | 0.4698 mL | 0.9397 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet