UBE2I Human His
目录号 : GP22358Ubiquitin-Conjugating Enzyme E2I Human Recombinant, His Tag
Sample solution is provided at 25 µL, 10mM.
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| Purity | Greater than 95.0% as determined by(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. | Source | Escherichia Coli. |
| Phycical Appearance | Sterile Filtered white lyophilized powder. | Shipping Condition | Shipped at Room temp. |
| Synonyms | SUMO-conjugating enzyme UBC9; EC 6.3.2.-; SUMO-protein ligase; Ubiquitin-conjugating enzyme E2 I; Ubiquitin-protein ligase I; Ubiquitin carrier protein I; Ubiquitin carrier protein 9; p18; UBC9; C358B7.1. | ||
| Amino Acid Sequence | MHHHHHHAMGTLNMSGIALSRLAQERKAWRKDHPFGFVAVPTKNPDGTMNLMNWECAIPGKKGTPWEGGLFKLRMLFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSILEEDKDWRPAITIKQILLGIQELLNEPNIQDPAQAEAYTIYCQNRVEYEKRVRAQAKKFAPS. | ||
| Solubility | It is recommended to reconstitute the lyophilized UBE2I in sterile water not less than 100µg/ml, which can then be further diluted to other aqueous solutions. | ||
| Stability | Lyophilized UBE2I although stable at room temperature for 3 weeks, should be stored desiccated below -18°C . Upon reconstitution UBE2I should be stored at 4°C between 2-7 days and for future use below -18°C .For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. | ||
| Formulation | Lyophilized from a 0.2ж╠m filtered concentrated (1mg/ml) solution in 1X PBS and 1mM DTT, pH 7.5. | ||
Human Ubquitin Conjugating Enzyme 9 (Ubc9) is a member of the E2 family and is specific for the conjugation of SUMO to a variety of target proteins. SUMO conjugation to target proteins is mediated by a different, but analogous, pathway to ubiquitinylation. This E2 is unusual in that it interacts directly with protein substrates that are modified by sumolyation, and may play a role in substrate recognition. Ubc9 can mediate the conjugation of SUMO-1 to a variety of proteins including RanGAP1, I?B?, and PML without the requirement of an E3 ligase.
Lyophilized UBE2I although stable at room temperature for 3 weeks, should be stored desiccated below -18°C . Upon reconstitution UBE2I should be stored at 4°C between 2-7 days and for future use below -18°C .For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.