UK 1
(Synonyms: 2'-(2-羟基苯基)-[2,4'-联苯并恶唑]-4-羧酸甲酯) 目录号 : GC12638
A cytotoxic Streptomyces metabolite
Cas No.:151271-53-3
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
UK 1, an unusual bis-benzoxazole metabolite isolated from Streptomyces sp. 517-02, is an inhibitor of topoisomerase II (Topo II) and hepatitis C viral replication. It is an antifungal and an antibiotic, and displays a wide spectrum of potent anticancer activities against lymphoma, leukemia, and certain solid tumor-derived cell lines with IC50 values as low as 20 nM. However, UK 1 is devoid of antimicrobial activity and is inactive against Pseudomonas aeruginosa and Staphylococcus aureus, a methicillin-resistant strain of S. aureus. Additionally, UK-1 can bind a wide variety of di- and tri-valent metal ions, particularly Mg2+ ions, and form complexes with DNA in the presence of Mg2+ ions. Topo II are ATP-dependent enzymes, which can simultaneously cut both strands of the DNA helix to manage DNA supercoils and tangles.
In vitro: UK 1 strongly blocked the growth of human epitheloid carcinoma HeLa, mouse melanoma B16, mouse leukemia P388 cells with ED50 values of 1.22 μg/ml, 1.17 μg/ml, and 0.11 μg/ml, respectively. UK 1 did not display any growth inhibitory activity against bacteria, fungi and yeasts up to 100 μg/ml [1].
In vivo: Up to now, in vivo study of UK 1 is still in the development stage.
Reference:
[1]. UEKI, M., UENO, K., MIYADOH, S., ABE, K., SHIBATA, K., TANIGUCHI, M., & OI, S. UK-1, a novel cytotoxic metabolite from Streptomyces sp. 517-02. I. Taxonomy, fermentation, isolation, physico-chemical and biological properties. The Journal of Antibiotics. 1993; 46(7): 1089-1094.
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 2.588 mL | 12.94 mL | 25.8799 mL |
| 5 mM | 0.5176 mL | 2.588 mL | 5.176 mL |
| 10 mM | 0.2588 mL | 1.294 mL | 2.588 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。