Uridine-5'-diphosphoglucuronic Acid (sodium salt)
(Synonyms: 尿苷二磷酸葡萄糖醛酸三钠) 目录号 : GC45128
Uridine-5'-diphosphoglucuronic Acid（钠盐）是 P2Y14 受体的激动剂。
Cas No.:63700-19-6
Sample solution is provided at 25 µL, 10mM.
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Uridine-5'-diphosphoglucuronic Acid (sodium salt) is an agonist for the P2Y14 receptor. Uridine-5'-diphosphoglucuronic Acid (sodium salt) is also a precursor of glucose-containing oligosaccharides, polysaccharides, glycoproteins, and glycolipids in animal tissues and certain microorganisms, and acts as an activated form of glucose in nucleotide sugar metabolism, serving as a substrate for glucose transferase.
Rat liver microsomes were incubated with 1 μM supinoxin.The addition of Uridine-5'-diphosphoglucuronic Acid (sodium salt) in the NADPH-containing incubation mixture did not lead to significant metabolic profile differences from the mixture containing only NADPH, indicating the negligible contribution of Uridine-5'-diphosphoglucuronic Acid (sodium salt)-dependent metabolism in the microsomal clearance of supinoxin[1]. Metabolic reactions containing FDU-PB-22 and human liver microsomes (HLMs) were independent of NADPH but not Uridine-5'-diphosphoglucuronic Acid (sodium salt) [2].
References:
[1]. Liu P, Wu S, et,al. Identification of the metabolites of tofacitinib in liver microsomes by liquid chromatography combined with high resolution mass spectrometry. Biomed Chromatogr. 2021 Jun;35(6):e5081. doi: 10.1002/bmc.5081. Epub 2021 Feb 10. PMID: 33522621.
[2]. Jones S, Yarbrough AL, et,al. Enzymatic analysis of glucuronidation of synthetic cannabinoid 1-naphthyl 1-(4-fluorobenzyl)-1H-indole-3-carboxylate (FDU-PB-22). Xenobiotica. 2019 Dec;49(12):1388-1395. doi: 10.1080/00498254.2019.1580403. Epub 2019 Mar 20. PMID: 30739533; PMCID: PMC7133092.
Uridine-5'-diphosphoglucuronic Acid（钠盐）是 P2Y14 受体的激动剂。 Uridine-5'-diphosphoglucuronic Acid (sodium salt) 也是动物组织和某些微生物中含葡萄糖的低聚糖、多糖、糖蛋白和糖脂的前体，在核苷酸糖代谢中作为葡萄糖的活化形式，作为葡萄糖转移酶底物。
大鼠肝微粒体与 1 μM supinoxin 一起孵育。在含有 NADPH 的孵育混合物中添加 Uridine-5'-diphosphoglucuronic Acid（钠盐）不会导致与仅含有 NADPH 的混合物的显着代谢特征差异，表明Uridine-5'-二磷酸葡萄糖醛酸（钠盐）依赖性代谢对 supinoxin 微粒体清除的贡献可忽略不计[1]。含有 FDU-PB-22 和人肝微粒体 (HLM) 的代谢反应不依赖于 NADPH，但不依赖于尿苷 5'-二磷酸葡萄糖醛酸（钠盐）[2]。
| Microsomal incubation experimen[1]: | |
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Material |
Rat liver microsomes |
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Preparation Method |
Rat liver microsomes were incubated with 1 μM supinoxin for 60 min in the presence and absence of (A) NADPH, (B) Uridine-5'-diphosphoglucuronic Acid (UDPGA), or (C) both NADPH and UDPGA as a cofactor. |
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Applications |
Rat liver microsomes were incubated with 1 μM supinoxin. The addition of UDPGA in the NADPH-containing incubation mixture did not lead to significant metabolic profile differences from the mixture containing only NADPH, indicating the negligible contribution of UDPGA-dependent metabolism in the microsomal clearance of supinoxin. |
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References: [1]. Liu P, Wu S, et,al. Identification of the metabolites of tofacitinib in liver microsomes by liquid chromatography combined with high resolution mass spectrometry. Biomed Chromatogr. 2021 Jun;35(6):e5081. doi: 10.1002/bmc.5081. Epub 2021 Feb 10. PMID: 33522621. |
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| Cas No. | 63700-19-6 | SDF | |
| 别名 | 尿苷二磷酸葡萄糖醛酸三钠 | ||
| Canonical SMILES | O=P(OP(OC[C@@H]1[C@@H](O)[C@@H](O)[C@H](N2C=CC(NC2=O)=O)O1)([O-])=O)([O-])O[C@H]3O[C@H](C([O-])=O)[C@@H](O)[C@H](O)[C@H]3O.[Na+].[Na+].[Na+] | ||
| 分子式 | C15H19N2O18P2•3Na | 分子量 | 646.2 |
| 溶解度 | H2O : 125 mg/mL (193.43 mM; Need ultrasonic); DMSO : 20 mg/mL (30.95 mM; Need ultrasonic (<50°C)) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.5475 mL | 7.7375 mL | 15.4751 mL |
| 5 mM | 0.3095 mL | 1.5475 mL | 3.095 mL |
| 10 mM | 0.1548 mL | 0.7738 mL | 1.5475 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet