Vialinin A
(Synonyms: Terrestrin A) 目录号 : GC17269A natural immunosuppressant from fungi
Cas No.:858134-23-3
Sample solution is provided at 25 µL, 10mM.
Vialinin A is a potent inhibitor of ubiquitin-specific peptidases with IC50 values of 1.5, 5.9 and 22.3 μM for ubiquitin-specific peptidase 4 (USP4), USP5/isopeptidase T (IsoT) and UCH-L1 deubiquitinating enzyme (DUB), respectively [1].
Ubiquitin-specific peptidases (USPs) are a series of proteases that deubiquitinate target proteins. The dynamic modification of a protein with ubiquitin can modify its function, localization and fate in the cell. Ubiquitin hydrolase plays an important role in immunology and infection [2].
In RBL-2H3 cells, vialinin A inhibited TNF-ɑ production and release and inhibited USP5 enzymatic activity. In USP5 siRNA-knockdown RBL-2H3 cells, vialinin A inhibited TNF-ɑ production and release in a dose dependent way. Also, the mRNA expression of TNF-ɑ was decreased significantly [2]. In RBL-2H3 cells, Vialinin A inhibited TNF-ɑ production with IC50 value of 0.09 nM. Also, it inhibited the release of interleukin 4, β-hexosaminidase and monocyte chemotactic protein 1, which suggested Vialinin A had anti-allergic activities [3].
References:
[1]. Okada K, Ye YQ, Taniguchi K, et al. Vialinin A is a ubiquitin-specific peptidase inhibitor. Bioorg Med Chem Lett, 2013, 23(15): 4328-4331.
[2]. Yoshioka Y, Ye YQ, Okada K, et al. Ubiquitin-specific peptidase 5, a target molecule of vialinin A, is a key molecule of TNF-α production in RBL-2H3 cells. PLoS One, 2013, 8(12): e80931.
[3]. Onose J, Xie C, Ye YQ, et al. Vialinin A, a novel potent inhibitor of TNF-alpha production from RBL-2H3 cells. Biol Pharm Bull, 2008, 31(5): 831-833.
Cas No. | 858134-23-3 | SDF | |
别名 | Terrestrin A | ||
化学名 | [3,4-dihydroxy-2,5-bis(4-hydroxyphenyl)-6-(2-phenylacetyl)oxyphenyl] 2-phenylacetate | ||
Canonical SMILES | C1=CC=C(C=C1)CC(=O)OC2=C(C(=C(C(=C2C3=CC=C(C=C3)O)O)O)C4=CC=C(C=C4)O)OC(=O)CC5=CC=CC=C5 | ||
分子式 | C34H26O8 | 分子量 | 562.57 |
溶解度 | 10mg/mL in ethanol, 20mg/mL in DMSO or in DMF | 储存条件 | Store at -20°C |
General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 储备液的保存方式和期限:-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。 为了提高溶解度,请将管子加热至37℃,然后在超声波浴中震荡一段时间。 |
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Shipping Condition | 评估样品解决方案:配备蓝冰进行发货。所有其他可用尺寸:配备RT,或根据请求配备蓝冰。 |
制备储备液 | |||
1 mg | 5 mg | 10 mg | |
1 mM | 1.7776 mL | 8.8878 mL | 17.7756 mL |
5 mM | 0.3555 mL | 1.7776 mL | 3.5551 mL |
10 mM | 0.1778 mL | 0.8888 mL | 1.7776 mL |
第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量) | ||||||||||
给药剂量 | mg/kg | 动物平均体重 | g | 每只动物给药体积 | ul | 动物数量 | 只 | |||
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系GLPBIO为您提供正确的澄清溶液配方) | ||||||||||
% DMSO % % Tween 80 % saline | ||||||||||
计算重置 |
计算结果:
工作液浓度: mg/ml;
DMSO母液配制方法: mg 药物溶于 μL DMSO溶液(母液浓度 mg/mL,
体内配方配制方法:取 μL DMSO母液,加入 μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入 μL saline,混匀澄清。
1. 首先保证母液是澄清的;
2.
一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
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- Purity: >98.00%
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