Vinyl-L-NIO (hydrochloride)
目录号 : GC12928A potent, selective nNOS inhibitor
Cas No.:728944-69-2
Sample solution is provided at 25 µL, 10mM.
IC50: 100 nM, 12 and 60 μM for nNOS, eNOS, and iNOS, respectively
Vinyl-L-NIO is a potent and selective inhibitor of nNOS.
Nitric oxide synthase (NOS) catalyzes the NADPH- and O2-dependent conversion of L-arginine to nitric oxide (NO) and citrulline. Three isoforms including the neuronal (nNOS), endothelial, and inducible have currently been identified. Since NO overproduction is able to contribute to various pathophysiological conditions, NOS inhibitors are considered as potential therapeutic agents.
In vitro: Vinyl-L-NIO was identified as a potent and selective inhibitor of nNOS. The Ki values for inhibition of nNOS, eNOS, and iNOS are 100 nM, 12 and 60 μM, respectively, as determined using initial rate measurements. Moreove, vinyl-L-NIO could irreversibly inactivate nNOS with a kinact of 0.078 min-1 and a Ki value of 90 nM in the presence of NADPH and O2. In additioin, it was found that vinyl-L-NIO was not able to inactivate iNOS and eNOS needed 20-fold higher concentrations of vinyl-L-NIO to achive 75% the rate of inactivation seen with nNOS [1].
In vivo: Vinyl-L-NIO was intracerebroventricularly injected at a dose of 10 microg/rat just before intraperitoneal injection of LPS. Vinyl-L-NIO injected at a selected doses had no effect on normal day-time body temperature and normal night-time. Vinyl-L-NIO at a dose of 10 microg/animal could suppress the LPS-induced fever in rats. The fever index calculated for rats pretreated with vinyl-L-NIO was reduced by 43%, compared to that calculated for water-pretreated and LPS-injected rats [2].
Clinical trial: So far, no clinical study has been conducted.
References:
[1] Babu, B. R., and Griffith, O.W. N5-(1-Imino-3-butenyl)-L-ornithine. A neuronal isoform selective mechanism-based inactivator of nitric oxide synthase. The Journal of Biological Chemisty 273, 8882-8889 (1998).
[2] Soszynski D, Chelminiak M. Intracerebroventricular injection of neuronal and inducible nitric oxide synthase inhibitors attenuates fever due to LPS in rats. J Physiol Pharmacol. 2007 Sep;58(3):551-61.
Cas No. | 728944-69-2 | SDF | |
化学名 | N5-(1-imino-3-butenyl)-L-ornithine, monohydrochloride | ||
Canonical SMILES | C=CCC(NCCC[C@H](N)C(O)=O)=N.Cl | ||
分子式 | C9H17N3O2 • HCl | 分子量 | 235.7 |
溶解度 | ≤30mg/ml in ethanol;50mg/ml in DMSO;50mg/ml in dimethyl formamide | 储存条件 | Store at -20°C |
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Shipping Condition | 评估样品解决方案:配备蓝冰进行发货。所有其他可用尺寸:配备RT,或根据请求配备蓝冰。 |
制备储备液 | |||
1 mg | 5 mg | 10 mg | |
1 mM | 4.2427 mL | 21.2134 mL | 42.4268 mL |
5 mM | 0.8485 mL | 4.2427 mL | 8.4854 mL |
10 mM | 0.4243 mL | 2.1213 mL | 4.2427 mL |
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给药剂量 | mg/kg | 动物平均体重 | g | 每只动物给药体积 | ul | 动物数量 | 只 | |||
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% DMSO % % Tween 80 % saline | ||||||||||
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工作液浓度: mg/ml;
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2.
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Quality Control & SDS
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- Purity: >98.00%
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