Vitamin K1-d7
(Synonyms: 维生素K1-d7) 目录号 : GC46235
An internal standard for the quantification of vitamin K1
Cas No.:1233937-39-7
Sample solution is provided at 25 µL, 10mM.
;)
,-1-7$$$37316672.jpg');)
;)
;)
$$$36806353.jpg');)
;33(7)%EF%BC%9A546-561$$$37156877.jpg');)
;)
;)
;)
%201124-1138.$$$35908550.jpg');)
%20766-780.$$$37100057.jpg');)
%20418-432.$$$36893736.jpg');)
%EF%BC%9A-240-255.$$$35108512.jpg');)
533-545$$$36543525.jpg');)
%201-13.$$$36600053.jpg');)
;)
Quality Control & SDS
- View current batch:
- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Vitamin K1-d7 is intended for use as an internal standard for the quantification of vitamin K1 by GC- or LC-MS. Vitamin K1 is a fat-soluble, dietary nutrient that is essential for the synthesis of proteins important for blood-clotting, bone metabolism, and cell growth.1 It is found in the photosynthetic tissues of green, leafy plants, where it acts as an electron acceptor forming part of the electron transport chain of Photosystem I.2 Vitamin K1 also serves as a precursor to vitamin K2 and is reported to exhibit anticancer activity in various cell lines.3,4
|1. Plaza, S.M., and Lamson, D.W. Vitamin K2 in bone metabolism and osteoporosis. Altern. Med. Rev. 10(1), 24-35 (2005).|2. Santabarbara, S., Heathcote, P., and Evans, M.C.W. Modelling of the electron transfer reactions in Photosystem I by electron tunnelling theory: The phylloquinones bound to the PsaA and the PsaB reaction centre subunits of PS I are almost isoenergetic to the iron-sulfur cluster FX. Biochim. Biophys. Acta. 1708(3), 283-310 (2005).|3. Beulens, J.W.J., Booth, S.L., van den Heuvel, E.G.H.M., et al. The role of menaquinones (vitamin K2) in human health. Br. J. Nutr. 1-12 (2013).|4. Lamson, D.W., and Plaza, S.M. The anticancer effects of vitamin K. Altern. Med. Rev. 8(3), 303-318 (2003).
| Cas No. | 1233937-39-7 | SDF | |
| 别名 | 维生素K1-d7 | ||
| Canonical SMILES | O=C1C(C/C=C(C)/CCCC(C)CCCC(C)CCCC(C)C)=C(C([2H])([2H])[2H])C(C2=C([2H])C([2H])=C([2H])C([2H])=C21)=O | ||
| 分子式 | C31H39D7O2 | 分子量 | 457.7 |
| 溶解度 | DMSO : ≥ 25 mg/mL (54.62 mM); Ethanol : ≥ 25 mg/mL (54.62 mM); DMF : ≥ 25 mg/mL (54.62 mM) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
||
| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 2.1848 mL | 10.9242 mL | 21.8484 mL |
| 5 mM | 0.437 mL | 2.1848 mL | 4.3697 mL |
| 10 mM | 0.2185 mL | 1.0924 mL | 2.1848 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Routine Supercritical Fluid Chromatography Tandem Mass Spectrometry Method for Determination of Vitamin K1 Extracted from Serum with a 96-Well Solid-Phase Extraction Method
J Appl Lab Med 2017 May 1;1(6):637-648.PMID:33379828DOI:10.1373/jalm.2016.021717
Background: The concentration of vitamin K1 in serum or plasma is the most common index for assessing vitamin K status. The aim of this study was to develop and validate a rapid and reliable routine method for quantifying vitamin K1 above 0.1 ng/mL. Semi-automation of a simple sample preparation with fast analysis by supercritical fluid chromatography-tandem mass spectrometry (SFC-MS/MS) was exploited. Methods: Vitamin K1 was extracted from 250-μL serum samples by the use of protein precipitation and reversed-phase solid-phase extraction (SPE) in 96-well plates and quantified by SFC on a 2.1 × 100 mm Torus 1-Aminoanthracene (1-AA) column in 3.8 min with electrospray ionization-tandem mass spectrometry (MS/MS) detection. Results: This method shows good linearity in the concentration range of 0.1-50 ng/mL with a correlation coefficient of R2 >0.999. Imprecision was satisfactory, with repeatability and reproducibility <10% CV. The lower limit of the measuring interval was 0.1 ng/mL, and no systematic bias was observed for the method, which used Vitamin K1-d7 as internal standard. Recovery of vitamin K1 in external quality controls was satisfactory compared to other laboratories participating in the external quality assurance scheme. The method is currently in routine use for analysis of serum samples. Conclusions: The method allows high-throughput reliable determination of vitamin K1 in serum in the range 0.1-50 ng/mL.