WaTx (36-68) (scorpion) (trifluoroacetate salt)
(Synonyms: Wasabi Receptor Toxin (36-68)) 目录号 : GC91865Wasabi receptor toxin (WaTx) (36-68) is a cell-penetrating peptide that corresponds to the mature toxin sequence and is an activator of transient receptor potential ankyrin 1 (TRPA1).
Sample solution is provided at 25 µL, 10mM.
Quality Control & SDS
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- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Wasabi receptor toxin (WaTx) (36-68) is a cell-penetrating peptide that corresponds to the mature toxin sequence and is an activator of transient receptor potential ankyrin 1 (TRPA1).[1] It selectively induces currents in HEK293T cells expressing human TRPA1 (EC50 = 15 nM) over cells expressing human TRP melastatin 8 (TRPM8) or human TRP vanilloid 1 (TRPV1) at 100 nM or rat voltage-gated potassium channel 1.2 (Kv1.2), rat Kv2.1, rat Kv3.1, or rat Kv4.3 at 1,000 nM. WaTx induces nocifensive behavior in wild-type but not Trpa1-/- mice.
References:
[1].King, J.V.L., Emrick, J.J., Kelly, M.J.S., et al.A cell-penetrating scorpion toxin enables mode-specific modulation of TRPA1 and painCell178(6)1362-1374(2019).
制备储备液 | |||
1 mg | 5 mg | 10 mg | |
1 mM | 0.2594 mL | 1.2969 mL | 2.5938 mL |
5 mM | 0.0519 mL | 0.2594 mL | 0.5188 mL |
10 mM | 0.0259 mL | 0.1297 mL | 0.2594 mL |
第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量) | ||||||||||
给药剂量 | mg/kg | 动物平均体重 | g | 每只动物给药体积 | ul | 动物数量 | 只 | |||
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系GLPBIO为您提供正确的澄清溶液配方) | ||||||||||
% DMSO % % Tween 80 % saline | ||||||||||
计算重置 |
计算结果:
工作液浓度: mg/ml;
DMSO母液配制方法: mg 药物溶于 μL DMSO溶液(母液浓度 mg/mL,
体内配方配制方法:取 μL DMSO母液,加入 μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入 μL saline,混匀澄清。
1. 首先保证母液是澄清的;
2.
一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。