Wedelolactone
(Synonyms: 蟛蜞菊内酯) 目录号 : GN10269
A natural NF-κB inhibitor
Cas No.:524-12-9
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >99.50%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Wedelolactone is an inhibitor of IKK.
IkB kinase (IKK) complex contains the catalytic subunits IKKα and IKKβ, and the regulatory subunit IKKγ/NEMO. IKK, is a kinase critical for activation of NF-κB by mediating phosphorylation and degradation of IκBα. The induction of caspase-11 is an important upstream controlling event in inflammatory response and apoptosis under pathological conditions modulated by upstream NF-κB-mediated transcription[1].
In vitro: In cultured mouse BALB/c 3T3 cells, mouse splenocytes and HeLa cells, wedelolactone (0~100 μM) inhibited LPS-induced caspase-11 expression by inhibiting NF-κB-mediated transcription through the direct inhibition of IKK. Wedelolactone played an potential role in anti-inflammatory therapy to inhibit IL-1β levels in diseases such as rheumatoid arthritis, asthma and septic shock[1].
In vivo: On Swiss albino male mouse skin, IKK inhibition by wedelolactone (10 μM) prevented the induction of NF-κB, perturbed the generation of reactive oxygen species and reactive nitrogen intermediates, blunted the signal transduction that lead to the activation of the early immediate genes, and thereby protected mouse skin from the UVB induced neoplastic transformation, angiotropism and metastatic progression [2].
References:
[1] Kobori M, Yang Z, Gong D, et al. Wedelolactone suppresses LPS-induced caspase-11 expression by directly inhibiting the IKK complex.[J]. Cell Death & Differentiation, 2004, 11(1):123-30.
[2] Ali F, Khan B A, Sultana S. Wedelolactone mitigates UVB induced oxidative stress, inflammation and early tumor promotion events in murine skin: plausible role of NFκB pathway[J]. European Journal of Pharmacology, 2016, 786:253-264.
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.1822 mL | 15.9109 mL | 31.8218 mL |
| 5 mM | 0.6364 mL | 3.1822 mL | 6.3644 mL |
| 10 mM | 0.3182 mL | 1.5911 mL | 3.1822 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。