X1 compound
目录号 : GC26082X1 compound是非编码RNA原型Xist的RepA基序的高亲和力结合物，Kd值为0.4±0.3μM
Sample solution is provided at 25 µL, 10mM.
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X1 compound is a high-affinity binder to RepA motif of non-coding RNA prototype Xist with a Kd of 0.4±0.3μM. X1 compound shows progressively disruptive effects on RepA-PRC2 and RepA-SPEN interactions, with IC50 values of 30μM and 48μM, respectively and blocks initiation of X-chromosome inactivation. X1 compound exhibits inhibitory effects on cell growth and differentiation at a concentration of 5μM[1].
In vitro, 10 μM X1 compound treated female embryoid bodies (EB) derived from mouse ES cells with two active X chromosomes for 1-5 days inhibited cell differentiation and growth, with RepA-PRC2 and RepA-SPEN interaction inhibition observed on day2, and poor EB outgrowth, blunted H3K27me3 enrichment, and X-chromosome inactivation seen after 72 hours[1].
References:
[1] Aguilar R, Spencer KB, Kesner B,et al. Targeting Xist with compounds that disrupt RNA structure and X inactivation. Nature. 2022 Apr;604(7904):160-166
X1 compound是非编码RNA原型Xist的RepA基序的高亲和力结合物，Kd值为0.4±0.3μM。X1 compound对RepA-PRC2和RepA-SPEN的相互作用表现出抑制作用，IC50值分别为30μM和48μM，并阻断X染色体失活。X1 compound达到5μM时就可以表现出对细胞生长和分化的抑制作用[1]。
在体外，10μM X1 compound处理携带活性X染色体的小鼠ES细胞的雌性胚状体（EB）1-5天，细胞分化和生长受到抑制，第2天观察到RepA-PRC2和RepA-SPEN相互作用被抑制，72小时后观察到EB生长不良，H3K27me3富集减弱，X染色体失活[1]。
| Cell experiment [1]: | |
Cell lines | Mouse embryonic stem cells |
Preparation Method | The female mouse embryonic stem (ES) cell model (TsixTST/+) initially carry two active X chromosome’s (Xa) but recapitulate X-chromosome inactivation when induced to differentiate into embryoid bodies (EB) for 4 days. The differentiation process was conducted with 10μM X1 compound on day 2 then the exposure was prolonged to day 7. |
Reaction Conditions | 10µM; 1-5 days |
Applications | X1 compound treatment resulted in a female-specific inhibition of cell differentiation and growth by reducing RepA–PRC2 and RepA-SPEN interaction and blocking initiation of X-chromosome inactivation. |
References: | |
| Cas No. | SDF | Download SDF | |
| 分子式 | C27H20N4O | 分子量 | 416.48 |
| 溶解度 | DMSO: 83 mg/mL (199.29 mM);Water: Insoluble;Ethanol: 20 mg/mL (48.02 mM) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.4011 mL | 12.0054 mL | 24.0108 mL |
| 5 mM | 0.4802 mL | 2.4011 mL | 4.8022 mL |
| 10 mM | 0.2401 mL | 1.2005 mL | 2.4011 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet