Xylan
(Synonyms: 木聚糖) 目录号 : GC30220
Xylan是开花植物的次生植物细胞壁中主要的半纤维素成分。Xylan是一种由木糖单元组成的多糖，主要包含由纤维素连接的β-D-木糖单位。
Cas No.:9014-63-5
Sample solution is provided at 25 µL, 10mM.
;)
,-1-7$$$37316672.jpg');)
;)
;)
$$$36806353.jpg');)
;33(7)%EF%BC%9A546-561$$$37156877.jpg');)
;)
;)
;)
%201124-1138.$$$35908550.jpg');)
%20766-780.$$$37100057.jpg');)
%20418-432.$$$36893736.jpg');)
%EF%BC%9A-240-255.$$$35108512.jpg');)
533-545$$$36543525.jpg');)
%201-13.$$$36600053.jpg');)
;)
Quality Control & SDS
- View current batch:
- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
|
Kinase experiment: |
Arabinoglucuronoxylan (AGX) is extracted and purified from Norway spruce (Picea abies) secondary cell walls using two complementary processes: (i) alkali extraction after delignification (AGX-A) and (ii) subcritical water extraction and enzymatic purification (AGX-H). The monosaccharide composition of the Xylan is analyzed after acid methanolysis (1 M TFA, 3h, 100°C) and high-pH anion-exchange chromatography with pulsed amperometric detection[1]. |
|
References: [1]. Martinez-Abad A, et al. Regular Motifs in Xylan Modulate Molecular Flexibility and Interactions with Cellulose Surfaces. Plant Physiol. 2017 Oct 25. pii: pp.01184.2017. |
|
Xylan represents the main hemicellulose component in the secondary plant cell walls of flowering plants. Xylan is a polysaccharide made from units of xylose and contains predominantly β-D-xylose units linked as in cellulose.
Two different Xylan fractions are extracted from spruce wood using an alkaline process (AGX-A) and a hydrothermal process by subcritical water (AGX-H). AGX-A shows lower relative glucuronation and higher arabinosylation compare with AGX-H. These differences in substitution may arise from the different extractability of distinct Xylan populations in softwoods or be induced by the extraction process. mGlcA substitutions do not affect significantly the conformational space of the Xylan backbone, neither in their protonated nor deprotonated state[1].
[1]. Martinez-Abad A, et al. Regular Motifs in Xylan Modulate Molecular Flexibility and Interactions with Cellulose Surfaces. Plant Physiol. 2017 Oct 25. pii: pp.01184.2017.
| Cas No. | 9014-63-5 | SDF | |
| 别名 | 木聚糖 | ||
| Canonical SMILES | [Xylan] | ||
| 分子式 | 分子量 | ||
| 溶解度 | DMSO : 100 mg/mL ;Water : ≥ 33.33 mg/mL | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
||
| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Xylan biosynthesis
Curr Opin Biotechnol 2014 Apr;26:100-7.24679265 10.1016/j.copbio.2013.11.013
Plant cells are surrounded by a rigid wall made up of cellulose microfibrils, pectins, hemicelluloses, and lignin. This cell wall provides structure and protection for plant cells. In grasses and in dicot secondary cell walls, the major hemicellulose is a polymer of β-(1,4)-linked xylose units called Xylan. Unlike cellulose--which is synthesized by large complexes at the plasma membrane--xylan is synthesized by enzymes in the Golgi apparatus. Xylan synthesis thus requires the coordinated action and regulation of these synthetic enzymes as well as others that synthesize and transport substrates into the Golgi. Recent research has identified several genes involved in Xylan synthesis, some of which have already been used in engineering efforts to create plants that are better suited for biofuel production.
Outstanding questions on Xylan biosynthesis
Plant Sci 2022 Dec;325:111476.36174800 10.1016/j.plantsci.2022.111476
Xylan is the second most abundant polysaccharide in plant biomass. It is a crucial component of cell wall structure as well as a significant factor contributing to biomass recalcitrance. Xylan consists of a linear chain of β-1,4-linked xylosyl residues that are often substituted with glycosyl side chains, such as glucuronosyl/methylglucuronosyl and arabinofuranosyl residues, and acetylated at O-2 and/or O-3. Xylan from gymnosperms and dicots contains a unique reducing end tetrasaccharide sequence that is not detected in Xylan from grasses, bryophytes and seedless vascular plants. Grass Xylan is heavily decorated at O-3 with arabinofuranosyl residues that are frequently esterified with hydroxycinnamates. Genetic and biochemical studies have uncovered a number of genes involved in Xylan backbone elongation and acetylation, Xylan glycosyl substitutions and their modifications, and the synthesis of the unique Xylan reducing end tetrasaccharide sequence, but some outstanding issues on the biosynthesis of Xylan still remain unanswered. Here, we provide a brief overview of Xylan structure and focus on discussion of the current understanding and open questions on Xylan biosynthesis. Further elucidation of the biochemical mechanisms underlying Xylan biosynthesis will not only shed new insights into cell wall biology but also provide molecular tools for genetic modification of biomass composition tailored for diverse end uses.
Xylan in drug delivery: A review of its engineered structures and biomedical applications
Eur J Pharm Biopharm 2020 Jun;151:199-208.32339636 10.1016/j.ejpb.2020.04.016
Xylan, an abundant biopolymer mainly extracted from plants and algae, is commonly studied for textile, food and biomedical applications. In this review, different approaches to obtain xylan-based products for drug delivery purposes were described. Investigations about xylan-based films, micro- and nanostructure, with the ability or not to swell (hydrogels), developed for biomedical applications, were summarized. Furthermore, a section on colon drug delivery and the methods that have been developed for the evaluation of these systems were presented.
Balanced Xylan Acetylation is the Key Regulator of Plant Growth and Development, and Cell Wall Structure and for Industrial Utilization
Int J Mol Sci 2020 Oct 23;21(21):7875.33114198 PMC7660596
Xylan is the most abundant hemicellulose, constitutes about 25-35% of the dry biomass of woody and lignified tissues, and occurs up to 50% in some cereal grains. The accurate degree and position of Xylan acetylation is necessary for Xylan function and for plant growth and development. The post synthetic acetylation of cell wall Xylan, mainly regulated by Reduced Wall Acetylation (RWA), Trichome Birefringence-Like (TBL), and Altered Xyloglucan 9 (AXY9) genes, is essential for effective bonding of Xylan with cellulose. Recent studies have proven that not only Xylan acetylation but also its deacetylation is vital for various plant functions. Thus, the present review focuses on the latest advances in understanding Xylan acetylation and deacetylation and explores their effects on plant growth and development. Baseline knowledge about precise regulation of Xylan acetylation and deacetylation is pivotal to developing plant biomass better suited for second-generation liquid biofuel production.
Gut microbial utilization of Xylan and its implication in gut homeostasis and metabolic response
Carbohydr Polym 2022 Jun 15;286:119271.35337525 10.1016/j.carbpol.2022.119271
Xylan as the second most abundant indigestible carbohydrate found in nature attracts great interests of researchers, nutritionist and consumers due to its various health benefits. However, accumulated studies indicate the interactions with gut microbiota greatly affect these benefits, and significant progress has been made over the past few years to understand how microbes utilize Xylan at gene level. In this review, we focused on gut xylanolytic microbes and Xylan's physico-chemical features, summarized the xylanases needed for complete Xylan decomposition, their substrate specificity and the presence in gut microbes, as well as microbial degradation of Xylan in single strain mode and cooperation mode. Xylan utilization system were discussed with different phyla. Furthermore, the implications on intestinal homeostasis and metabolic response were reviewed with clinical effects emphasized, and highlight is placed on specific gut microbes and the complexity of Xylan structure to provide a clue for the inconsistent results in human studies. CHEMICAL COMPOUNDS: Xylan; arabinoxylan, glucuronoxylans; glucuronoarabinoxylans; xylo-oligosaccharides; arabinoxylo-oligosaccharides.