Palmitoleic acid
(Synonyms: 棕榈油酸；(Z)-Hexadec-9-enoic acid) 目录号 : GC31253
Palmitoleic acid是一种omega-7单不饱和脂肪酸，在澳洲坚果植物中含量很高。
Cas No.:373-49-9
Sample solution is provided at 25 µL, 10mM.
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Palmitoleic acid is an omega-7 monounsaturated fatty acid that is found in high concentrations in macadamia nuts[1]. Palmitoleic acid has anti-inflammatory properties and can improve insulin sensitivity in the liver and skeletal muscle[2, 3]. Dietary supplementation with palmitoleic acid can prevent cardiovascular disease and diet-induced metabolic disorders[4].
In vitro, treatment of EAHy926 cells with Palmitoleic acid (1-500μM) for 48h significantly inhibited cell viability and reduced the production of proinflammatory cytokines stimulated by TNFα at doses greater than 200μM[5]. Treatment of hepatocyte cell lines (HepG2, HuH7, and WRL68 cells) with Palmitoleic acid (660μM) for 24h significantly induced cell apoptosis and inhibited insulin receptor downstream signaling[6].
In vivo, topical treatment of skin wounds in Wistar rats with 100μL Palmitoleic acid (100μM) for 15 days significantly accelerated wound closure, reduced the area of injury, and decreased L-selectin concentrations at the wound site[7].
References:
[1] Hu W, Fitzgerald M, Topp B, et al. A review of biological functions, health benefits, and possible de novo biosynthetic pathway of palmitoleic acid in macadamia nuts[J]. Journal of Functional Foods, 2019, 62: 103520.
[2] Souza C O, Teixeira A A S, Biondo L A, et al. Palmitoleic acid reduces high fat diet-induced liver inflammation by promoting PPAR-γ-independent M2a polarization of myeloid cells[J]. Biochimica et Biophysica Acta (BBA)-Molecular and Cell Biology of Lipids, 2020, 1865(10): 158776.
[3] Mozaffarian D, Cao H, King I B, et al. Circulating palmitoleic acid and risk of metabolic abnormalities and new-onset diabetes[J]. The American journal of clinical nutrition, 2010, 92(6): 1350-1358.
[4] Yang Z H, Pryor M, Noguchi A, et al. Dietary palmitoleic acid attenuates atherosclerosis progression and hyperlipidemia in low‐density lipoprotein receptor‐deficient mice[J]. Molecular nutrition & food research, 2019, 63(12): 1900120.
[5] de Souza C O, Valenzuela C A, Baker E J, et al. Palmitoleic acid has stronger anti‐inflammatory potential in human endothelial cells compared to oleic and palmitic acids[J]. Molecular nutrition & food research, 2018, 62(20): 1800322.
[6] Ricchi M, Odoardi M R, Carulli L, et al. Differential effect of oleic and palmitic acid on lipid accumulation and apoptosis in cultured hepatocytes[J]. Journal of gastroenterology and hepatology, 2009, 24(5): 830-840.
[7] Weimann E, Silva M B B, Murata G M, et al. Topical anti-inflammatory activity of palmitoleic acid improves wound healing[J]. PloS one, 2018, 13(10): e0205338.
Palmitoleic acid是一种omega-7单不饱和脂肪酸，在澳洲坚果植物中含量很高[1]。Palmitoleic acid具有抗炎作用，能够提高肝脏和骨骼肌的胰岛素敏感性[2, 3]。在膳食中补充Palmitoleic acid能够预防心血管疾病和饮食引起的代谢紊乱[4]。
在体外，Palmitoleic acid（1-500μM）处理EAHy926细胞48h，剂量大于200μM情况下显著抑制了细胞活力，减少了TNFα刺激产生的促炎细胞因子[5]。Palmitoleic acid（660μM）处理肝细胞系（HepG2、HuH7、WRL68细胞）24h，显著诱导了细胞凋亡，抑制了胰岛素受体下游信号传导[6]。
在体内，采用100μL Palmitoleic acid（100μM）局部治疗Wistar大鼠皮肤上的伤口15天，显著加速了伤口闭合，减少了受伤面积，降低了伤口部位的L-选择素浓度[7]。
| Cell experiment [1]: | |
Cell lines | EAHy926 cells |
Preparation Method | EAHy926 cells were incubated with DMEM or different concentrations (1, 5, 10, 20, 50, 100, 200, and 500μM) of Palmitic acid, Oleic acid, or Palmitoleic acid for 48h and then incubated with or without TNFα (1ng/mL) for another 24h. Cell viability was determined using the MTT assay. |
Reaction Conditions | 1, 5, 10, 20, 50, 100, 200, 500μM; 48h |
Applications | Palmitoleic acid significantly reduced cell viability at concentrations above 200μM. |
| Animal experiment [2]: | |
Animal models | Wistar rats |
Preparation Method | Animals were initially anaesthetized using ketamine (60mg/kg) and xylazine (10mg/kg), after which a 10mm2 piece of skin was surgically removed from the dorsal region of each rat. After surgery, the wound was treated topically with 100μL of Palmitoleic acid (100μM) or the same volume of sterile phosphate-buffered saline. The rats were maintained in individual cages under a warming lamp and were monitored until fully recovered from the anaesthesia. Palmitoleic acid or PBS was administered once daily, the 15th day was the end point of this experiment. |
Dosage form | 100μM, Topical treatment of wounds for 15 days |
Applications | Palmitoleic acid directly hastened the wound closure. Integration of curves representing the wound closure area monitored over 12 days demonstrated that treating the wound with Palmitoleic acid decreased the wounded area compared with the untreated group. |
References: | |
| Cas No. | 373-49-9 | SDF | |
| 别名 | 棕榈油酸；(Z)-Hexadec-9-enoic acid | ||
| Canonical SMILES | CCCCCC/C=C\CCCCCCCC(O)=O | ||
| 分子式 | C16H30O2 | 分子量 | 254.41 |
| 溶解度 | ≤50mg/ml in ethanol;30mg/ml in DMSO;30mg/ml in dimethyl formamide | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.9307 mL | 19.6533 mL | 39.3066 mL |
| 5 mM | 0.7861 mL | 3.9307 mL | 7.8613 mL |
| 10 mM | 0.3931 mL | 1.9653 mL | 3.9307 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet