ZK 93423 hydrochloride
目录号 : GC13922
GABAA receptor agonist
Cas No.:1216574-52-5
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
IC50: 1 nM
ZK-93423 is an anxiolytic drug from the β-Carboline family, which is a nonbenzodiazepine GABAA receptor agonist. The GABAA receptor is an ligand-gated ion channel and ionotropic receptor.
In vitro: The full agonist ZK-93423 is not subtype selective and stimulates α1, α2, α3, and α5-subunit containing GABAA receptors equally [α1β32 (Ki = 4.1 nM), α2β32 (Ki = 4.2 nM), α3β32 (Ki = 6.0 nM), α5β32 (Ki = 4.5 nM), α6β32 (Ki >1000 nM)] [1]. ZK-93423 has also been used as a base to develop new and improved beta-carboline derivatives and help map the binding site of the GABAA receptor. In rats trained to discriminate PTZ from saline, the PTZ cue was antagonized by ZK 93423, which indicated ZK 93423 may exhibit anxiolytic quality [2].
In vivo: ZK 93423 showd to have anxiolytic properties on animal models of anxiety, including social interaction, vogel, geller serfter, 4-plate test and elevated plus-maze. The anxiogenic and anxiolytic actions are mediated by the BDZ binding sites [3].
Clinical trial: So far, no clinical study has been conducted.
References:
[1] Cox ED, Diaz-Arauzo H, Huang Q, Reddy MS, Ma C, Harris B, McKernan R, Skolnick P, Cook JM. Synthesis and evaluation of analogues of the partial agonist 6-(propyloxy)-4-(methoxymethyl)-beta-carboline-3-carboxylic acid ethyl ester (6-PBC) and the full agonist 6-(benzyloxy)-4-(methoxymethyl)-beta-carboline-3-carboxylic acid ethyl ester (Zk 93423) at wild type and recombinant GABAA receptors. J Med Chem. 1998 Jul 2;41(14):2537-52.
[2] Stephens DN, Shearman GT, Kehr W. Discriminative stimulus properties of beta-carbolines characterized as agonists and inverse agonists at central benzodiazepine receptors. Psychopharmacology (Berl). 1984;83(3):233-9.
[3] File SE, Baldwin HA. Effects of beta-carbolines in animal models of anxiety. Brain Res Bull. 1987 Sep;19(3):293-9.
| Cas No. | 1216574-52-5 | SDF | |
| 化学名 | ethyl 6-(benzyloxy)-4-(methoxymethyl)-9H-pyrido[3,4-b]indole-3-carboxylate | ||
| Canonical SMILES | CCOC(C1=NC=C2C(C3=C(N2)C=CC(OCC4=CC=CC=C4)=C3)=C1COC)=O | ||
| 分子式 | C23H22N2O4.HCl | 分子量 | 426.9 |
| 溶解度 | <11.71mg/ml in DMSO | 储存条件 | Desiccate at RT |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 2.3425 mL | 11.7123 mL | 23.4247 mL |
| 5 mM | 0.4685 mL | 2.3425 mL | 4.6849 mL |
| 10 mM | 0.2342 mL | 1.1712 mL | 2.3425 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。